Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; Department of Neurology, Xuan Wu Hospital, Capital Medical University, Beijing, China.
Innovation Center for Neurological Disorders, Xuan Wu Hospital, Capital Medical University, China; School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
Stem Cell Res. 2022 Apr;60:102690. doi: 10.1016/j.scr.2022.102690. Epub 2022 Jan 31.
Mutations in the APP gene are popularly known as the second cause trigger the familial Alzheimer's disease (AD). We generated a human induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells isolated from an AD patient using non-integrative Sendai virus. The iPSC line highly expresses pluripotency markers, has the capacity to differentiate into the normal teratoma tissue, retains the APP mutation, and displays the normal karyotype. The iPSC line will provide a useful resource to study the pathogenesis and drugs screening of AD.
APP 基因突变通常被认为是引发家族性阿尔茨海默病（AD）的第二大原因。我们使用非整合性仙台病毒从 AD 患者外周血单核细胞中生成了一个人诱导多能干细胞（iPSC）系。该 iPSC 系高度表达多能性标志物，具有分化为正常畸胎瘤组织的能力，保留了 APP 突变，并显示正常核型。该 iPSC 系将为研究 AD 的发病机制和药物筛选提供有用的资源。
