College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China; Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China.
Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China.
Stem Cell Res. 2024 Sep;79:103501. doi: 10.1016/j.scr.2024.103501. Epub 2024 Jul 15.
Apolipoprotein E (APOE)is the gene with greatest genetic risk for Alzheimer's disease (AD). We successfully established a human induced pluripotent stem cell(iPSC) line from a woman mutated by APOE gene. The cell line was isolated from this woman's peripheral blood mononuclear cells using a non-integrated Sendai virus, which retained the original genotype, showed a normal karyotype, highly expressed pluripotent markers and could differentiate into three germ layers.
载脂蛋白 E（APOE）是阿尔茨海默病（AD）最大的遗传风险基因。我们成功地从一位 APOE 基因突变的女性建立了一个人类诱导多能干细胞（iPSC）系。该细胞系使用非整合性的仙台病毒从该女性的外周血单个核细胞中分离出来，保留了原始基因型，具有正常核型，高度表达多能标志物，并能分化为三个胚层。
