Innovation Center for Neurological Disorders and Department of Neurology, Xuanwu Hospital, Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing, China.
College of Integrated Traditional Chinese and Western Medicine, Changchun University of Chinese Medicine, Changchun, China.
Stem Cell Res. 2023 Sep;71:103149. doi: 10.1016/j.scr.2023.103149. Epub 2023 Jun 21.
The β-amyloid precursor protein (APP) is a crucial pathogenic gene linked to Alzheimer's disease (AD). A human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) isolated from a female with APP gene mutation utilizing non-integrative Sendai virus. The iPSC line exhibits high expression of pluripotency markers, retains the APP mutation, displays a normal karyotype, and has the ability to differentiate into normal teratoma tissue. This iPSC line represents a valuable cell model for investigating the pathological mechanisms and therapeutic strategies of AD.
β-淀粉样前体蛋白（APP）是与阿尔茨海默病（AD）相关的关键致病基因。我们从一位携带 APP 基因突变的女性外周血单核细胞（PBMC）中利用非整合型仙台病毒生成了一个人诱导多能干细胞（iPSC）系。该 iPSC 系高表达多能性标志物，保留 APP 突变，具有正常核型，并能分化为正常畸胎瘤组织。该 iPSC 系是研究 AD 病理机制和治疗策略的有价值的细胞模型。
