Child Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, China.
Prenatal Diagnosis Center, Shijiazhuang Obstetrics and Gynecology Hospital, Key Laboratory of Maternal and Fetal Medicine of Hebei Province, Shijiazhuang, Hebei, 050011, China.
Stem Cell Res. 2023 Jun;69:103116. doi: 10.1016/j.scr.2023.103116. Epub 2023 May 6.
The ε4 allele of the lipoprotein E gene (APOE4) is the strongest genetic risk factor associated with sporadic Alzheimer's disease (sAD). While the neuronal cell type-specific function of APOE4 in connection with AD pathology remains understudied. Therefore, we generated an induced pluripotent stem cells (iPSCs) line from a 77-year-old female donor with ApoE4 genetic background. We reprogrammed peripheral blood mononuclear cells (PBMCs) with non-integrative Sendai viral vectors containing reprogramming factors. Established iPSCs showed the capability of pluripotency, three-germ differentiation in vitro with normal karyotype. Hence, the generated iPSC could be a powerful tool to conduct further studies of AD mechanisms.
载脂蛋白 E 基因（APOE4）的 ε4 等位基因是与散发性阿尔茨海默病（sAD）相关的最强遗传风险因素。虽然 APOE4 在与 AD 病理相关的神经元细胞类型特异性功能仍未得到充分研究。因此，我们从一位 77 岁的女性供体中生成了携带 ApoE4 遗传背景的诱导多能干细胞（iPSC）系。我们使用含有重编程因子的非整合性仙台病毒载体对外周血单核细胞（PBMCs）进行重编程。建立的 iPSC 显示出多能性的能力，能够在体外进行三胚层分化，且具有正常核型。因此，生成的 iPSC 可以成为研究 AD 机制的有力工具。
