Parks L W, Rodriguez R J, Low C
Lipids. 1986 Jan;21(1):89-91. doi: 10.1007/BF02534308.
Concentrations of ergosterol as low as 1.2 mM are effective in satisfying the essential high specificity microrequirement for C5,6 unsaturated sterol in yeast. In a sterol auxotroph supplemented with saturated sterol (cholestanol), aqueous extracts of yeast provide a growth factor that eliminates the ergosterol microrequirement. The factor is easily differentiated from ergosterol by solubility, thermostability, and thin layer and liquid chromatography and is functional at levels equivalent to those of vitamins, hormones and pheromones.
麦角固醇浓度低至1.2 mM时，就能有效满足酵母中对C5,6不饱和固醇必不可少的高特异性微量需求。在添加了饱和固醇（胆甾烷醇）的固醇营养缺陷型酵母中，酵母水提取物提供了一种生长因子，可消除对麦角固醇的微量需求。该因子很容易通过溶解度、热稳定性、薄层色谱和液相色谱与麦角固醇区分开来，其功能水平与维生素、激素和信息素相当。
