Center of Biomedical Research, Research Center for Human Disease Modeling, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
J Vet Med Sci. 2022 Apr 15;84(4):574-581. doi: 10.1292/jvms.21-0494. Epub 2022 Feb 11.
Bordetella bronchiseptica (B. bronchiseptica) is associated with respiratory tract infections in laboratory animals. In our laboratory animal facility, B. bronchiseptica was isolated from 21 of 27 apparently healthy rabbits obtained from a breeding farm contaminated with B. bronchiseptica. Restriction fragment length polymorphism (RFLP) analysis showed that the flagellin genotype of isolates from the laboratory animal facility and breeding farm was type A, which is seen relatively frequently in rabbits in Europe. To examine its pathogenicity, guinea pigs, rats, and mice were inoculated intranasally with a representative strain isolated in the laboratory animal facility. Following inoculation of 10 colony forming unit (cfu), severe inflammation was observed in the lungs of guinea pig and mice, although the inflammation was less severe in rats. The strain was recovered from the trachea and lungs of these species after inoculation with lower dose such as 10 or 10 cfu. These results suggest that the isolated strain causes respiratory tract infection in guinea pigs, rats, and mice, and that its pathogenicity higher in mice than in rats. This study extends our knowledge of interpreting the microbiologic status of laboratory animals, which will contribute to the development of reliable and reproducible animal experiments.
支气管败血波氏杆菌（Bordetella bronchiseptica，B. bronchiseptica）与实验动物的呼吸道感染有关。在我们的实验动物设施中，从 27 只来自受支气管败血波氏杆菌污染的繁殖场的看似健康的兔子中分离出了 21 株 B. bronchiseptica。限制性片段长度多态性（RFLP）分析显示，来自实验动物设施和繁殖场的分离株的鞭毛基因型为 A 型，这在欧洲的兔子中相对常见。为了研究其致病性，用从实验动物设施中分离的代表性菌株对豚鼠、大鼠和小鼠进行了鼻腔接种。接种 10 个菌落形成单位（cfu）后，豚鼠和小鼠的肺部出现严重炎症，而大鼠的炎症则较轻。在接种较低剂量（如 10 或 10 cfu）后，该菌株从这些物种的气管和肺部中被回收。这些结果表明，分离株可引起豚鼠、大鼠和小鼠的呼吸道感染，并且其在小鼠中的致病性高于大鼠。本研究扩展了我们对实验动物微生物学状态的理解，这将有助于开发可靠和可重复的动物实验。
