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热烫和腌制过程对腌制肉豆蔻的乙醇酰基转移酶(AAT)活性、肉豆蔻醚含量及品质参数的影响

Effects of blanching and pickling process on the alcohol acyltransferase (AAT) activity, myristicin content and quality parameters of pickled nutmeg ().

作者信息

Rahman Nurain, Mahmood Kaiser, Kamilah Hanisah, Sulaiman Syazana, Ibrahim Muhammad, Ariffin Fazilah

机构信息

Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Penang Malaysia.

Department of Crop Sciences, Faculty of Agriculture and Forestry, Universiti Putra Malaysia, Bintulu Sarawak Campus, Nyabau Road, P.O Box 396, 97008 Bintulu, Sarawak Malaysia.

出版信息

J Food Sci Technol. 2022 Feb;59(2):542-551. doi: 10.1007/s13197-021-05038-z. Epub 2021 Feb 24.

Abstract

Myristicin, a potential toxicant, is a major ester present in the pericarp of nutmeg () and alcohol acyltransferase (AAT) is responsible for its generation. The objective of the study was firstly to estimate the effect of blanching and pickling process on the inactivation of AAT and production of myristicin in nutmeg pericarp. Secondly, the effect of pickling process on the physical, microbial, antioxidant and sensory properties of nutmeg was evaluated. The nutmeg fruit pericarp was water-blanched for 0, 3, 5, 7 and 9 min. The increased blanching time significantly ( < 0.05) reduced the AAT activity (3.478 U/mg protein) compared to fresh nutmeg (39.034 U/mg protein). The reduction of myristicin was so efficient that it could not be detected after 9 min of blanching. Similarly, the pickling process significantly ( < 0.05) inhibited the AAT activity and lowered the myristicin content. However, the blanching significantly ( < 0.05) lowered the total phenol content and reduced the free radical scavenging capacity of pickled nutmeg (BP) when compared with commercial pickled nutmeg. Blanched pickled nutmeg (BP) presented the same color characteristics as of commercial one, although significant reduction in hardness was observed. No growth of yeast and mold was noticed for all the blanched pickled nutmeg. The sensory analysis data demonstrated that blanching up to 7 min maintained the overall acceptability of pickled nutmeg as of commercial pickled nutmeg. Thus, the study suggests that blanching and pickling treatment improved the quality of nutmeg effectively by inhibiting the AAT activity and reducing the myristicin content.

摘要

肉豆蔻醚是一种潜在的有毒物质,是肉豆蔻果皮中存在的主要酯类,而乙醇酰基转移酶(AAT)负责其生成。本研究的目的首先是评估热烫和腌制过程对肉豆蔻果皮中AAT失活和肉豆蔻醚产生的影响。其次,评估腌制过程对肉豆蔻物理、微生物、抗氧化和感官特性的影响。将肉豆蔻果实的果皮进行0、3、5、7和9分钟的水烫处理。与新鲜肉豆蔻(39.034 U/mg蛋白质)相比,热烫时间的增加显著(P<0.05)降低了AAT活性(3.478 U/mg蛋白质)。肉豆蔻醚的减少非常有效,热烫9分钟后无法检测到。同样,腌制过程显著(P<0.05)抑制了AAT活性并降低了肉豆蔻醚含量。然而,与市售腌制肉豆蔻相比,热烫显著(P<0.05)降低了总酚含量并降低了腌制肉豆蔻(BP)的自由基清除能力。热烫腌制肉豆蔻(BP)呈现出与市售产品相同的颜色特征,尽管观察到硬度显著降低。所有热烫腌制肉豆蔻均未发现酵母和霉菌生长。感官分析数据表明,热烫7分钟以内的腌制肉豆蔻保持了与市售腌制肉豆蔻相同的总体可接受性。因此,该研究表明,热烫和腌制处理通过抑制AAT活性和降低肉豆蔻醚含量有效地提高了肉豆蔻的品质。

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