Faculty of Pharmacy, Universitas Padjadjaran, Jl KM 21.5 Bandung-Sumedang, Jatinangor, Indonesia; E-Mails:
Int J Mol Sci. 2010 Nov 23;11(11):4771-81. doi: 10.3390/ijms11114771.
The present study was designed to evaluate the inhibitory effect of nutmeg (Myristica fragrans Houtt.) seed essential oil on the locomotor activity of mice in a wheel cage. Active compounds in the essential oil were identified by off-line solid phase extraction (SPE-C18) and GC/MS analysis. The essential oil was administered by inhalation at doses of 0.1, 0.3, and 0.5 mL/cage. The results showed that inhalation of nutmeg seed essential oil at a dose of 0.5 mL/cage decreased locomotion by 68.62%; and inhalation of 0.1 and 0.3 mL/cage inhibited locomotion by 62.81% and 65.33%, respectively. Generally, larger doses and longer administrations of nutmeg seed essential oil exhibited greater locomotor inhibition. Subsequently, the plasma concentrations of essential oil compounds were measured. The most concentrated compound in the plasma was myristicin. Half an hour after the addition of 1 mL/cage of nutmeg seed oil, the plasma concentration of myristicin was 3.7 μg/mL; one and two hours after the addition, the blood levels of myristicin were 5.2 μg/mL and 7.1 μg/mL, respectively. Other essential oil compounds identified in plasma were safrole (two-hour inhalation: 1.28 μg/mL), 4-terpineol (half-hour inhalation: 1.49 μg/mL, one-hour inhalation: 2.95 μg/mL, two-hour inhalation: 6.28 μg/mL) and fatty esters. The concentrations of the essential oil compounds in the blood plasma were relatively low (μg/mL or ppm). In conclusion, the volatile compounds of nutmeg seed essential oil identified in the blood plasma may correlate with the locomotor-inhibiting properties of the oil when administered by inhalation.
本研究旨在评估肉豆蔻（Myristica fragrans Houtt.）种子精油对笼中小鼠活动能力的抑制作用。通过离线固相萃取（SPE-C18）和 GC/MS 分析鉴定精油中的活性化合物。精油以 0.1、0.3 和 0.5 mL/笼的剂量通过吸入给药。结果表明，吸入 0.5 mL/笼的肉豆蔻种子精油可使运动减少 68.62%；而吸入 0.1 和 0.3 mL/笼分别抑制运动 62.81%和 65.33%。通常，较大剂量和较长时间的肉豆蔻种子精油给药表现出更大的运动抑制作用。随后，测量了精油化合物在血浆中的浓度。血浆中最集中的化合物是肉豆蔻醚。在加入 1 mL/笼肉豆蔻种子油半小时后，血浆中肉豆蔻醚的浓度为 3.7 μg/mL；加入后 1 小时和 2 小时，血液中的肉豆蔻醚浓度分别为 5.2 μg/mL 和 7.1 μg/mL。在血浆中鉴定出的其他精油化合物为黄樟素（2 小时吸入：1.28 μg/mL）、4-松油醇（半小时吸入：1.49 μg/mL、1 小时吸入：2.95 μg/mL、2 小时吸入：6.28 μg/mL）和脂肪酸酯。精油化合物在血浆中的浓度相对较低（μg/mL 或 ppm）。总之，在血浆中鉴定出的肉豆蔻种子精油挥发性化合物可能与通过吸入给药时油的运动抑制特性相关。
