Wang Baiyu, Hu Wenshun, Fang Yaxue, Feng Xiaoxi, Fang Jingping, Zou Tengyue, Zheng Shaoquan, Ming Ray, Zhang Jisen
Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Fujian Breeding Engineering Technology Research Center for Longan & Loquat, Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China.
Front Plant Sci. 2022 Jan 27;12:813798. doi: 10.3389/fpls.2021.813798. eCollection 2021.
Longan ( Lour.) is an important economic crop widely planted in tropical and subtropical regions, and flower and fruit development play decisive effects on the longan yield and fruit quality formation. MCM1, AGAMOUS, DEFICIENS, Serum Response Factor (MADS)-box transcription factor family plays important roles for the flowering time, floral organ identity, and fruit development in plants. However, there is no systematic information of MADS-box family in longan. In this study, 114 MADS-box genes were identified from the longan genome, phylogenetic analysis divided them into type I (α, β, γ) and type II (*, ) groups, and genes were further clustered into 12 subfamilies. Comparative genomic analysis of 12 representative plant species revealed the conservation of type II in Sapindaceae and analysis of cis-elements revealed that transcription factors might directly regulate the genes. An ABCDE model was proposed for longan based on the phylogenetic analysis and expression patterns of MADS-box genes. Transcriptome analysis revealed that genes showed wide expression spectrums, particularly in reproductive organs. From 35 days after KClO treatment, 11 genes were up-regulated, suggesting a crucial role in off-season flower induction, while , , , and may act as the inhibitors. The gene expression patterns of longan fruit development indicated that , , and could be involved in fruit growth and ripening. This paper carried out the whole genome identification and analysis of the longan MADS-box family for the first time, which provides new insights for further understanding its function in flowers and fruit.
龙眼（Lour.）是一种广泛种植于热带和亚热带地区的重要经济作物，其花和果实发育对龙眼产量和果实品质形成起着决定性作用。MCM1、AGAMOUS、DEFICIENS、血清反应因子（MADS）-盒转录因子家族在植物的开花时间、花器官特征和果实发育中发挥着重要作用。然而，龙眼MADS-盒家族尚无系统信息。本研究从龙眼基因组中鉴定出114个MADS-盒基因，系统发育分析将它们分为I型（α、β、γ）和II型（*、 ）组，且 基因进一步聚为12个亚家族。对12种代表性植物物种的比较基因组分析揭示了无患子科中II型的保守性，顺式元件分析表明 转录因子可能直接调控 基因。基于MADS-盒基因的系统发育分析和表达模式，提出了龙眼的ABCDE模型。转录组分析表明 基因表现出广泛的表达谱，尤其是在生殖器官中。在KClO处理后35天，11个 基因上调，表明其在反季节花诱导中起关键作用，而 、 、 和 可能起抑制作用。龙眼果实发育的基因表达模式表明， 、 和 可能参与果实生长和成熟。本文首次对龙眼MADS-盒家族进行了全基因组鉴定和分析，为进一步了解其在花和果实中的功能提供了新的见解。
