Badon A, Andrique L, Mombereau A, Rivet L, Boyreau A, Nassoy P, Recher G
LP2N, Laboratoire Photonique Numérique et Nanosciences, University Bordeaux, Talence 33400, France.
Institut d'Optique Graduate School and CNRS UMR 5298, Talence 33400, France.
R Soc Open Sci. 2022 Feb 9;9(2):211444. doi: 10.1098/rsos.211444. eCollection 2022 Feb.
Optical imaging has rapidly evolved in the last decades. Sophisticated microscopes allowing optical sectioning for three-dimensional imaging or sub-diffraction resolution are available. Due to price and maintenance issues, these microscopes are often shared between users in facilities. Consequently, long-term access is often prohibited and does not allow to monitor slowly evolving biological systems or to validate new models like organoids. Preliminary coarse long-term data that do not require acquisition of terabytes of high-resolution images are important as a first step. By contrast with expensive all-in-one commercialized stations, standard microscopes equipped with incubator stages offer a more cost-effective solution despite imperfect long-run atmosphere and temperature control. Here, we present the , a custom-made compact microscope that fits into a table-top incubator. It is cheap and simple to implement, user-friendly and yet provides high imaging performances. The system has a field of view of 5.5 × 8 mm, a 3 μm resolution, a 10 frames per second acquisition rate, and is controlled with a Python-based graphical interface. We exemplify the capabilities of the on biological applications such as the hatching of eggs, the growth of the slime mould and of encapsulated spheroids of mammalian cells.
在过去几十年中，光学成像技术发展迅速。现在已有复杂的显微镜，可进行光学切片以实现三维成像或亚衍射分辨率成像。由于价格和维护问题，这些显微镜在设施中常由用户共享。因此，长期使用通常是被禁止的，而且无法用于监测缓慢演变的生物系统或验证类器官等新模型。不需要获取数TB高分辨率图像的初步粗略长期数据作为第一步很重要。与昂贵的一体化商业化设备相比，配备培养箱载物台的标准显微镜尽管长期的环境和温度控制并不理想，但提供了更具成本效益的解决方案。在此，我们展示了一种定制的紧凑型显微镜，它可放入桌面式培养箱中。它价格便宜且易于安装，用户友好，同时具备高成像性能。该系统的视野为5.5×8毫米，分辨率为3微米，采集速率为每秒10帧，并通过基于Python的图形界面进行控制。我们通过生物应用举例说明了该显微镜的能力，如斑马鱼卵孵化、黏菌生长以及哺乳动物细胞包封球体的生长。
