Bazin Aymerick, Badon Amaury
LP2N, Laboratoire Photonique Numéerique et Nanosciences, Univ. Bordeaux, F-33400 Talence, France.
Institut d'Optique Graduate School & CNRS UMR 5298, F-33400 Talence, France.
Biomed Opt Express. 2025 Feb 13;16(3):1062-1070. doi: 10.1364/BOE.544498. eCollection 2025 Mar 1.
Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream of the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile, and configured for bright-field and epifluorescence modes. We demonstrate its performance with imaging of multi-cellular aggregates of various shapes and sizes.
在生物显微镜领域，实现具有微米级分辨率的快速、大规模体积成像一直是一项长期挑战。为应对这一挑战，我们报告了一种增强版的光场显微镜，它在相机上游集成了一个电动倾斜镜。根据扫描模式，视场和/或横向分辨率可得到极大提高。我们的显微镜技术简单、通用，并且配置了明场和落射荧光模式。我们通过对各种形状和大小的多细胞聚集体成像来展示其性能。
