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zIncubascope：培养箱内多细胞聚集体的长期定量成像。

zIncubascope: Long-term quantitative imaging of multi-cellular assemblies inside an incubator.

作者信息

Jana Anirban, Mekhileri Naveen, Boyreau Adeline, Bazin Aymerick, Pujol Nadège, Alessandri Kevin, Recher Gaëlle, Nassoy Pierre, Badon Amaury

机构信息

LP2N, Laboratoire Photonique Numérique et Nanosciences, University Bordeaux, Talence, France.

Institut d' Optique Graduate School & CNRS UMR 5298, Talence, France.

出版信息

PLoS One. 2025 Jan 23;20(1):e0309035. doi: 10.1371/journal.pone.0309035. eCollection 2025.

DOI:10.1371/journal.pone.0309035
PMID:39847558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11756754/
Abstract

Recent advances in bioengineering have made it possible to develop increasingly complex biological systems to recapitulate organ functions as closely as possible in vitro. Monitoring the assembly and growth of multi-cellular aggregates, micro-tissues or organoids and extracting quantitative information is a crucial but challenging task required to decipher the underlying morphogenetic mechanisms. We present here an imaging platform designed to be accommodated inside an incubator which provides high-throughput monitoring of cell assemblies over days and weeks. We exemplify the capabilities of our system by investigating human induced pluripotent stem cells (hiPSCs) enclosed in spherical capsules, hiPSCs in tubular capsules and yeast cells in spherical capsules. Combined with a customized pipeline of image analysis, our solution provides insight into the impact of confinement on the morphogenesis of these self-organized systems.

摘要

生物工程领域的最新进展使得开发日益复杂的生物系统成为可能，以便在体外尽可能逼真地模拟器官功能。监测多细胞聚集体、微组织或类器官的组装和生长并提取定量信息，是解读潜在形态发生机制所需的一项关键但具有挑战性的任务。我们在此展示一个设计用于放置在培养箱内的成像平台，该平台可对细胞组装体进行长达数天甚至数周的高通量监测。我们通过研究封装在球形胶囊中的人诱导多能干细胞（hiPSC）、管状胶囊中的hiPSC以及球形胶囊中的酵母细胞，来例证我们系统的功能。结合定制的图像分析流程，我们的解决方案深入洞察了限制条件对这些自组织系统形态发生的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/a22529861619/pone.0309035.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/389b77026f87/pone.0309035.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/5b5d7cba3a86/pone.0309035.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/fa144767d27b/pone.0309035.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/e2a02951a71d/pone.0309035.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/efc0165e14d8/pone.0309035.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/62b8ffdeea8a/pone.0309035.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/a22529861619/pone.0309035.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/389b77026f87/pone.0309035.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/144fef1b023a/pone.0309035.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/5b5d7cba3a86/pone.0309035.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/fa144767d27b/pone.0309035.g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33ee/11756754/a22529861619/pone.0309035.g008.jpg

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