Nilforoushzadeh Mohammad Ali, Behtash Amiri Amir, Shaghaghi Behrad, Alimohammadi Alimohammad, Ahmadi Rahim, Khodaverdi Darian Ebrahim, Razzaghi Mohammadreza, Rezaei Tavirani Mostafa, Zare Sona
Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Jordan Dermatology and Hair Transplantation Center, Tehran, Iran.
J Lasers Med Sci. 2021 Dec 1;12:e77. doi: 10.34172/jlms.2021.77. eCollection 2021.
Wound healing can have a very important impact on the patients' quality of life. For its treatment, wound dressings have vital and effective uses. Indeed, the use of a proper wound dressing can improve the healing process and duration. Recently, wound dressings with unique properties have been prepared using natural hydrogels. In addition to the general wound characteristics, new generations of wound dressings, such as those lasting longer on the wound, can have specific properties such as transferring allogeneic cells to enhance the healing effect and speed up the healing process. The present study aimed to prepare a gelatin-based hydrogel and to characterize it for therapeutic purposes. In this experimental-laboratory study, a gelatin hydrogel was made using a microbial transglutaminase (mTG) enzyme. The prepared hydrogel was evaluated in terms of appearance, physical, and chemical properties. To investigate the biological properties of the hydrogel, cells were cultured on it and the toxicity of the hydrogel for the cells was investigated. The location of the cells on the hydrogel was imaged via an electron microscope. The absorption and reflectance characteristics of the hydrogel were recorded by optical spectroscopy. Data were collected and statistical analysis was performed. The results showed that the mTG gelatin hydrogel had a uniform pore size and good physical, chemical, and mechanical properties for use in wound healing. Cell experiments showed evident cell proliferation and high viability. The results also revealed that the cells grew vigorously and adhered tightly to the hydrogel. The preparation of a gelatin hydrogel under GMP conditions can be considered in the healing of diabetic wounds and burns.
伤口愈合对患者的生活质量可能会产生非常重要的影响。对于其治疗，伤口敷料具有至关重要且有效的用途。事实上，使用合适的伤口敷料可以改善愈合过程和缩短愈合时间。最近，利用天然水凝胶制备了具有独特性能的伤口敷料。除了一般的伤口特性外，新一代的伤口敷料，如那些能在伤口上保持更长时间的敷料，还可具有特定性能，如转移同种异体细胞以增强愈合效果并加速愈合过程。本研究旨在制备一种基于明胶的水凝胶并对其进行治疗用途的表征。在这项实验性实验室研究中，使用微生物转谷氨酰胺酶（mTG）制备了明胶水凝胶。对制备的水凝胶在外观、物理和化学性质方面进行了评估。为了研究水凝胶的生物学特性，将细胞培养在其上并研究水凝胶对细胞的毒性。通过电子显微镜对水凝胶上细胞的位置进行成像。用水光谱法记录水凝胶的吸收和反射特性。收集数据并进行统计分析。结果表明，mTG明胶水凝胶具有均匀的孔径，在伤口愈合方面具有良好的物理、化学和机械性能。细胞实验显示出明显的细胞增殖和高活力。结果还表明，细胞生长旺盛并紧密粘附于水凝胶。在糖尿病伤口和烧伤的愈合中可以考虑在GMP条件下制备明胶水凝胶。
