Arjmand Babak, Safari-Alighiarloo Nahid, Razzaghi Mohhamadreza, Rezaei Tavirani Mostafa, Rostami Nejad Mohammad, Rezaei Tavirani Majid, Mansouri Vahid, Vafaee Reza
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2021 Dec 28;12:e87. doi: 10.34172/jlms.2021.87. eCollection 2021.
Excessive exposure to ultraviolet (UV) radiation may cause a variety of skin cancers and damage to the eye lens. The assessment of different aspects of UV damage has attracted researchers' interest. UV radiation to simple biological models such as of yeast family could help to find out different molecular changes resulting from radiation. The assessment and network analysis of gene expression data about yeast cells radiated by the UV laser was the aim of this study. The gene expression profiles of samples in the presence of the UV laser at 30 seconds radiation and 15 minutes' post-radiation time are compared with the control profiles. The significant expressed genes interacted and the central nodes and related biological terms were identified. The main connected component of the network including 427 nodes was analyzed and 11 central differentially expressed genes (DEGs) were determined. RPN11, UBI4, HSP82, and HSC82 as critical DEGs and "positive regulation of telomere maintenance" as a related biological term were introduced. The finding has provided a new perspective on laser application in the rejuvenation process. It seems that the laser can be used as a suitable agent against the aging process which is a limiting factor in human life.
过度暴露于紫外线（UV）辐射可能会导致多种皮肤癌以及对眼睛晶状体造成损害。对紫外线损伤不同方面的评估引起了研究人员的兴趣。将紫外线辐射应用于简单的生物模型，如酵母家族，有助于找出辐射导致的不同分子变化。本研究的目的是对紫外线激光辐射的酵母细胞的基因表达数据进行评估和网络分析。将紫外线激光在30秒辐射时和辐射后15分钟时存在情况下的样本基因表达谱与对照谱进行比较。确定显著表达的基因之间的相互作用，并识别中心节点和相关生物学术语。分析了包括427个节点的网络的主要连通组件，并确定了11个中心差异表达基因（DEG）。引入了RPN11、UBI4、HSP82和HSC82作为关键DEG，以及“端粒维持的正调控”作为相关生物学术语。这一发现为激光在年轻化过程中的应用提供了新的视角。似乎激光可以用作对抗衰老过程的合适试剂，而衰老过程是人类生命中的一个限制因素。
