The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 430079 Wuhan, China.
Biomed Res Int. 2022 Feb 4;2022:2885323. doi: 10.1155/2022/2885323. eCollection 2022.
This study is aimed at exploring the role of nuclear transporting factor 2 (NUTF2) in head and neck squamous cell carcinoma (HNSCC) based on The Cancer Genome Atlas (TCGA) database.
We obtained 528 HNSCC patients' clinical data from TCGA and performed expression level analysis of NUTF2. Gene Sets Enrichment Analysis (GSEA) was conducted to identify NUTF2-associated regulatory mechanisms in HNSCC. In addition, several other tools were used to enrich the regulatory network.
We found that NUTF2 was significantly upregulated ( < 0.001) in HNSCC. We then observed that higher NUTF2 is associated with poorer overall survival and disease-free survival. Further, by using Cox analyses, we determined high NUTF2 as an independent risk factor of predicting poorer overall survival. Tumor immune infiltration analysis revealed a significantly negative correlation between NUTF2 expression and the level of tumor infiltrated CD8 T cell and B cell, suggesting that NUTF2 may be involved in the immune regulation of HNSCC. Gene sets related to T/B cell receptor signaling pathways were differentially enriched based on the NUTF2 expression phenotype. KEGG pathways were used to show that NUTF2 may affect proliferation, differentiation, and immune response of T/B cell through regulating PI3K/AKT, NFB, MAPK, and Calcium signaling pathways.
NUTF2 might be a valuable biomarker for HNSCC and correlated with T/B cell receptor signaling pathway.
本研究旨在基于癌症基因组图谱（TCGA）数据库探讨核转运因子 2（NUTF2）在头颈部鳞状细胞癌（HNSCC）中的作用。
我们从 TCGA 获得了 528 例 HNSCC 患者的临床数据，并对 NUTF2 的表达水平进行了分析。采用基因集富集分析（GSEA）鉴定 NUTF2 与 HNSCC 相关的调控机制。此外，还使用了其他几种工具来丰富调控网络。
我们发现 NUTF2 在 HNSCC 中显著上调（<0.001）。我们观察到 NUTF2 表达水平越高，总生存率和无病生存率越低。进一步通过 Cox 分析，我们确定高 NUTF2 是预测总生存率较差的独立危险因素。肿瘤免疫浸润分析显示 NUTF2 表达与肿瘤浸润 CD8 T 细胞和 B 细胞水平呈显著负相关，表明 NUTF2 可能参与 HNSCC 的免疫调节。基于 NUTF2 表达表型，差异富集了与 T/B 细胞受体信号通路相关的基因集。KEGG 通路显示，NUTF2 可能通过调节 PI3K/AKT、NF-κB、MAPK 和钙信号通路影响 T/B 细胞的增殖、分化和免疫反应。
NUTF2 可能是 HNSCC 的一个有价值的生物标志物，与 T/B 细胞受体信号通路相关。
