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对大肠杆菌70S核糖体具有不同亲和力的苯丙氨酰 - tRNA构象异构体的相互转化。

The interconversion of conformers of phenylalanyl-tRNA with different affinity to 70S ribosomes of Escherichia coli.

作者信息

Kirillov S V, Odinzov V B

出版信息

Nucleic Acids Res. 1978 May;5(5):1501-14. doi: 10.1093/nar/5.5.1501.

DOI:10.1093/nar/5.5.1501
PMID:351562
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342099/
Abstract

Earlier the existence of two conformers of Phe-tRNAPhe of E. coli was demonstrated because one of them yields complexes with 70S-poly(U) of extremely high affinity and the other with at least a 105 lower binding constant. We denote the first conformer as HAC (high affinity conformer) and the second as LAC (low affinity conformer). This high difference in binding constants was used for studying the process of reversible interconversion of conformers of Phe-tRNAPhe. The transition kinetics of LAC to HAC in conditions when the latter is stable (in the presence of magnesium ions) was studied and a high value of activation energy (35 kcal/mole) found. The interconversion is the first order reaction and equilibrium does not depend of overall Phe-tRNA concentration.

摘要

早些时候已证明大肠杆菌的苯丙氨酰 - tRNA苯丙氨酸存在两种构象异构体，因为其中一种能与70S - 聚（U）形成具有极高亲和力的复合物，而另一种的结合常数至少低105倍。我们将第一种构象异构体记为HAC（高亲和力构象异构体），第二种记为LAC（低亲和力构象异构体）。结合常数的这种巨大差异被用于研究苯丙氨酰 - tRNA苯丙氨酸构象异构体的可逆相互转化过程。研究了在后者稳定（存在镁离子）的条件下LAC向HAC的转变动力学，并发现了较高的活化能值（35千卡/摩尔）。这种相互转化是一级反应，且平衡不依赖于苯丙氨酰 - tRNA的总浓度。

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Nucleic Acids Res. 1982 Apr 10;10(7):2419-37. doi: 10.1093/nar/10.7.2419.
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Study on conformational states of Escherichia coli tRNAPhe in solution by a modulation-free ESR-spectrometer.利用无调制电子自旋共振光谱仪对溶液中大肠杆菌苯丙氨酸转运核糖核酸构象状态的研究。
Nucleic Acids Res. 1982 Feb 11;10(3):1113-26. doi: 10.1093/nar/10.3.1113.

本文引用的文献

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RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
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Two interconvertible forms of tryptophanyl sRNA in E. coli.大肠杆菌中色氨酰化小RNA的两种可相互转化形式。
Proc Natl Acad Sci U S A. 1966 Apr;55(4):948-56. doi: 10.1073/pnas.55.4.948.
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Tertiary structure in transfer ribonucleic acids.转运核糖核酸中的三级结构。
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A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.一种新的转运RNA片段反应：与核糖体-信使RNA复合物结合的TpψpCpGp诱导四磷酸鸟苷和五磷酸鸟苷的合成。
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The elongation reactions in protein synthesis.蛋白质合成中的延伸反应。
Adv Protein Chem. 1973;27:213-42. doi: 10.1016/s0065-3233(08)60448-9.
6
Specific recognition of GTpsiC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli.来自大肠杆菌的50S核糖体亚基对tRNA的GTpsiC环（环IV）的特异性识别。
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The involvement of 5S RNA in the binding of tRNA to ribosomes.5S RNA参与转运RNA与核糖体的结合。
Biochem Biophys Res Commun. 1973 Oct 1;54(3):942-8. doi: 10.1016/0006-291x(73)90785-7.
8
Tritium exchange studies of transfer RNA in native and denaturated conformations.天然和变性构象下转运RNA的氚交换研究。
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tRNA structure and binding sites for cations.
Biopolymers. 1972;11(7):1317-33. doi: 10.1002/bip.1972.360110702.
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Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.2.5埃分辨率下酵母苯丙氨酸转运RNA的结构
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4414-8. doi: 10.1073/pnas.72.11.4414.

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