一种新的转运RNA片段反应：与核糖体-信使RNA复合物结合的TpψpCpGp诱导四磷酸鸟苷和五磷酸鸟苷的合成。
A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.
作者信息
Richter D, Erdmann V A, Sprinzl M
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3226-9. doi: 10.1073/pnas.71.8.3226.
Abstract
The tetranucleotide TpPsipCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The TpPsipCpGp-dependent fragment reaction has a strict requirement for the 50S and 30S ribosomal subunits and synthetic or natural messenger RNA.
摘要
四核苷酸TpPsipCpGp与大肠杆菌50S核糖体亚基结合时，在严格因子指导下合成鸟苷四磷酸和五磷酸的过程中可替代完整的tRNA。依赖TpPsipCpGp的片段反应对50S和30S核糖体亚基以及合成或天然信使RNA有严格要求。
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A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.一种新的转运RNA片段反应：与核糖体-信使RNA复合物结合的TpψpCpGp诱导四磷酸鸟苷和五磷酸鸟苷的合成。
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本文引用的文献
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Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp.密码子特异性、依赖tRNA的体外合成ppGpp和pppGpp。
Nat New Biol. 1973 May 2;243(122):13-5.
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Nucleotide sequence of KB cell 5S RNA.KB细胞5S核糖核酸的核苷酸序列。
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Rapid ion-exchange chromatographic microanalysis of ultraviolet-absorbing materials and its application to nucleosides.紫外吸收物质的快速离子交换色谱微分析及其在核苷中的应用。
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Separation of large quantities of ribosomal subunits by zonal ultracentrifugation.通过区带超速离心法分离大量核糖体亚基。
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Ribosome-catalyzed peptidyl transfer: substrate specificity at the P-site.核糖体催化的肽基转移：P位点的底物特异性
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Specific recognition of GTpsiC loop (loop IV) of tRNA by 50S ribosomal subunits from E. coli.来自大肠杆菌的50S核糖体亚基对tRNA的GTpsiC环（环IV）的特异性识别。
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The involvement of 5S RNA in the binding of tRNA to ribosomes.5S RNA参与转运RNA与核糖体的结合。
Biochem Biophys Res Commun. 1973 Oct 1;54(3):942-8. doi: 10.1016/0006-291x(73)90785-7.
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Proc Natl Acad Sci U S A. 1973 Jul;70(7):2145-8. doi: 10.1073/pnas.70.7.2145.
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Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes.鸟苷四磷酸和五磷酸的合成需要在核糖体的受体位点存在密码子特异性的、未负载的转运核糖核酸。
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Nature. 1972 Aug 18;238(5364):381-4. doi: 10.1038/238381a0.
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