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利用无调制电子自旋共振光谱仪对溶液中大肠杆菌苯丙氨酸转运核糖核酸构象状态的研究。

Study on conformational states of Escherichia coli tRNAPhe in solution by a modulation-free ESR-spectrometer.

作者信息

Bondarev G N, Isaev-Ivanov V V, Isaeva-Ivanova L S, Kirillov S V, Kleiner A R, Lepekhin A F, Odinzov V B, Fomichev V N

出版信息

Nucleic Acids Res. 1982 Feb 11;10(3):1113-26. doi: 10.1093/nar/10.3.1113.

DOI:10.1093/nar/10.3.1113
PMID:6278435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326226/
Abstract

A modulation free Electron Spin Resonance spectrometer was used for the registration of spectral absorption lines of a spin-labeled Escherichia coli phenylalanine tRNA in solution with low (less than 0.1%) line shape distortion. The analysis of line shape of two different spin-labels introduced into position 8 revealed that phenylalanine tRNA in solution exists as a mixture of two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and functional state of tRNA (deacylated, aminoacylated or peptidylated). There are no overall structural rearrangements upon aminoacylation or peptidylation of tRNA. The observed small changes of spectral line shape can be assigned to shifts in conformational equilibria.

摘要

使用一台无调制电子自旋共振光谱仪记录溶液中自旋标记的大肠杆菌苯丙氨酸tRNA的光谱吸收线，其线形畸变率低（小于0.1%）。对引入第8位的两种不同自旋标记的线形分析表明，溶液中的苯丙氨酸tRNA以两种构象异构体的混合物形式存在，构象异构体之间的平衡取决于pH值、镁离子浓度和tRNA的功能状态（去酰化、氨酰化或肽酰化）。tRNA氨酰化或肽酰化后没有整体结构重排。观察到的光谱线形的微小变化可归因于构象平衡的移动。

相似文献

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Study on conformational states of Escherichia coli tRNAPhe in solution by a modulation-free ESR-spectrometer.利用无调制电子自旋共振光谱仪对溶液中大肠杆菌苯丙氨酸转运核糖核酸构象状态的研究。
Nucleic Acids Res. 1982 Feb 11;10(3):1113-26. doi: 10.1093/nar/10.3.1113.
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[Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation].
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Fluorescence-monitored conformational change on the 3'-end of tRNA upon aminoacylation.氨酰化作用下tRNA 3'端的荧光监测构象变化
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本文引用的文献

1
Conformational states of yeast tRNA Phe in the complex with cognate and non cognate synthetases.酵母苯丙氨酸tRNA与同源和非同源合成酶复合物中的构象状态。
Nucleic Acids Res. 1981 Feb 25;9(4):1031-44. doi: 10.1093/nar/9.4.1031.
2
Fluorimetric study of the complex between yeast phenylalanyl-tRNA synthetase and tRNA-Phe. 1. Changes in the conformation of the enzyme and tRNA; modification of the Wybutine neighbourhood.酵母苯丙氨酰 - tRNA合成酶与tRNA - Phe复合物的荧光研究。1. 酶和tRNA构象的变化；怀丁碱邻域的修饰。
Eur J Biochem. 1980 Jan;103(1):145-53. doi: 10.1111/j.1432-1033.1980.tb04298.x.
3
Interaction of 70 S ribosomes from Escherichia coli with spin-labeled N-Cbz-Phe-tRNAPhe. An electron paramagnetic resonance study.大肠杆菌70 S核糖体与自旋标记的N-苄氧羰基苯丙氨酰-tRNA苯丙氨酸的相互作用。一项电子顺磁共振研究。
J Biol Chem. 1980 Sep 10;255(17):8116-20.
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Improvement of the spin-labelling technique by the use of a radioactive spin label.通过使用放射性自旋标记改进自旋标记技术。
FEBS Lett. 1973 Nov 1;36(3):351-4. doi: 10.1016/0014-5793(73)80408-9.
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1H nuclear magnetic resonance of modified bases of valine transfer ribonucleic acid (Escherichia coli). A direct monitor of sequential thermal unfolding.缬氨酸转移核糖核酸（大肠杆菌）修饰碱基的1H核磁共振。序列热解链的直接监测。
Biochemistry. 1975 Aug 12;14(16):3612-8. doi: 10.1021/bi00687a015.
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Proton magnetic resonance studies on the conformation of the hexanucleotide, GmpApApYpApsiP, and Related fragments from the anticodong loop of baker's yeast phenylalanine transfer ribonucleic acid.关于六核苷酸GmpApApYpApsiP以及来自面包酵母苯丙氨酸转移核糖核酸反密码子环的相关片段构象的质子磁共振研究。
Biochemistry. 1975 Jul 15;14(14):3278-91. doi: 10.1021/bi00685a038.
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A speculation on the origin of protein synthesis.关于蛋白质合成起源的一种推测。
Orig Life. 1976 Dec;7(4):389-97. doi: 10.1007/BF00927934.
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Evidence for a conformational change in tRNAPhe upon aminoacylation.苯丙氨酸转运RNA氨酰化后构象变化的证据。
J Biol Chem. 1976 Mar 10;251(5):1529-30.
9
On the structure and conformational dynamics of yeast phenylalanine-accepting transfer ribonucleic acid in solution.溶液中酵母苯丙氨酸接受转移核糖核酸的结构与构象动力学
Biochemistry. 1979 Oct 16;18(21):4588-99. doi: 10.1021/bi00588a020.
10
The mechanism of codon-anticodon interaction in ribosomes. Heterogeneity of tRNA complexes with 70-S ribosomes of Escherichia coli.核糖体中密码子-反密码子相互作用的机制。大肠杆菌70-S核糖体与tRNA复合物的异质性。
Eur J Biochem. 1978 Aug 15;89(1):305-13. doi: 10.1111/j.1432-1033.1978.tb20928.x.

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