Frontiers Science Center for Flexible Electronics (FSCFE), Institute of Flexible Electronics (IFE), MIIT Key Laboratory of Flexible Electronics (KLoFE), Xi'an Key Laboratory of Special Medicine and Health Engineering, Northwestern Polytechnical University, Xi'an, China.
North Sichuan Medical College, Nanchong, China.
Crit Rev Biotechnol. 2023 May;43(3):415-432. doi: 10.1080/07388551.2022.2030295. Epub 2022 Feb 13.
While the research field and industrial market of diagnosis (IVD) thrived during and post the COVID-19 pandemic, the development of isothermal nucleic acid amplification test (INAAT) based rapid diagnosis was engendered in a global wised large measure as a problem-solving exercise. This review systematically analyzed the recent advances of INAAT strategies with practical case for the real-world scenario virus detection applications. With the qualities that make INAAT systems useful for making diagnosis relevant decisions, the key performance indicators and the cost-effectiveness of enzyme-assisted methods and enzyme-free methods were compared. The modularity of nucleic acid amplification reactions that can lead to thresholding signal amplifications using INAAT reagents and their methodology design were examined, alongside the potential application with rapid test platform/device integration. Given that clinical practitioners are, by and large, unaware of many the isothermal nucleic acid test advances. This review could bridge the arcane research field of different INAAT systems and signal output modalities with end-users in clinic when choosing suitable test kits and/or methods for rapid virus detection.
虽然诊断（IVD）的研究领域和工业市场在 COVID-19 大流行期间和之后蓬勃发展，但等温核酸扩增测试（INAAT）的快速诊断的发展在很大程度上是作为一种全球性的解决问题的手段而产生的。本文系统地分析了 INAAT 策略的最新进展，并结合实际案例分析了病毒检测应用的现实情况。INAAT 系统在做出相关诊断决策方面具有有用的特性，因此比较了酶辅助方法和无酶方法的关键性能指标和成本效益。还检查了核酸扩增反应的模块性，这些反应可以使用 INAAT 试剂进行阈值信号扩增，以及它们的方法设计，以及与快速测试平台/设备集成的潜在应用。鉴于临床医生对许多等温核酸测试进展知之甚少。因此，当选择适合快速病毒检测的试剂盒和/或方法时，本综述可以将不同 INAAT 系统和信号输出方式的神秘研究领域与临床的最终用户联系起来。
