酵母苯丙氨酸转运RNA（tRNAPhe）反密码子区域的一种新型构象变化。
A novel conformational change of the anticodon region of tRNAPhe (yeast).
作者信息
Urbanke C, Maass G
出版信息
Nucleic Acids Res. 1978 May;5(5):1551-60. doi: 10.1093/nar/5.5.1551.
Abstract
The temperature dependence of the fluorescence of the Y-base of tRNAPhe (yeast) was investigated kinetically by the temperature jump method. In the range between -15 degrees C and +30 degrees C A NOVEL CONFORMATIONAL TRANSITION OF THE TRNA could be characterized. This conformational change was found in the absence of any artificial label; it is a characteristic property of tRNAPhe in its native structure. This transition accounts for 30% of the total fluorescence change. Its activation enthalpy is 16 kcal/mole (67 kJ/mole), and the transition enthalpy is between -2 kcal/mole and +2 kcal/mole (+/-8 kJ/mole). A model is represented in which this transition can be explained by a a change in the stacking pattern of the anticodon loop. The experimental findings are discussed with respect to several hypotheses about the molecular mechanism of protein biosynthesis which postulate conformational rearrangements of the anticodon loop.
摘要
采用温度跃升法对酵母苯丙氨酸转运核糖核酸（tRNAPhe）中Y碱基荧光的温度依赖性进行了动力学研究。在-15℃至+30℃的温度范围内，可以表征出tRNA的一种新型构象转变。这种构象变化是在没有任何人工标记的情况下发现的；它是天然结构中tRNAPhe的一个特征属性。这种转变占总荧光变化的30%。其活化焓为16千卡/摩尔（67千焦/摩尔），转变焓在-2千卡/摩尔至+2千卡/摩尔（±8千焦/摩尔）之间。提出了一个模型，其中这种转变可以通过反密码子环堆积模式的变化来解释。结合关于蛋白质生物合成分子机制的几个假设对实验结果进行了讨论，这些假设假定了反密码子环的构象重排。
相似文献
1
A novel conformational change of the anticodon region of tRNAPhe (yeast).酵母苯丙氨酸转运RNA（tRNAPhe）反密码子区域的一种新型构象变化。
Nucleic Acids Res. 1978 May;5(5):1551-60. doi: 10.1093/nar/5.5.1551.
2
Minor conformational changes of yeast tRNAPhe anticodon loop occur upon aminoacylation as indicated by Y base fluorescence.如Y碱基荧光所示，酵母苯丙氨酸转运核糖核酸（tRNAPhe）反密码子环在氨酰化时会发生微小的构象变化。
J Biochem. 1981 May;89(5):1439-43. doi: 10.1093/oxfordjournals.jbchem.a133336.
3
Fluorescence detected circular dichroism study of the anticodon loop of yeast tRNAPhe.酵母苯丙氨酸转运核糖核酸反密码子环的荧光检测圆二色性研究
Biochemistry. 1975 Aug 26;14(17):3794-9. doi: 10.1021/bi00688a011.
4
Proton nuclear magnetic resonance of minor nucleosides in yeast phenylalanine transfer ribonucleic acid. Conformational changes as a consequence of aminoacylation, removal of the Y base, and codon--anticodon interaction.酵母苯丙氨酸转移核糖核酸中稀有核苷的质子核磁共振。氨基酰化、Y碱基去除以及密码子-反密码子相互作用导致的构象变化。
Biochemistry. 1979 Jul 24;18(15):3189-99. doi: 10.1021/bi00582a001.
5
On the structure and conformational dynamics of yeast phenylalanine-accepting transfer ribonucleic acid in solution.溶液中酵母苯丙氨酸接受转移核糖核酸的结构与构象动力学
Biochemistry. 1979 Oct 16;18(21):4588-99. doi: 10.1021/bi00588a020.
6
Anticodon loop of tRNAPhe: structure, dynamics, and Mg2+ binding.苯丙氨酸转运RNA的反密码子环：结构、动力学及镁离子结合
Biochemistry. 1986 Oct 21;25(21):6365-71. doi: 10.1021/bi00369a004.
7
The conformation of the tRNAPhe anticodon loop monitored by fluorescence.通过荧光监测的苯丙氨酸转运RNA反密码子环的构象。
Nucleic Acids Res. 1984 Feb 24;12(4):2157-70. doi: 10.1093/nar/12.4.2157.
8
An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNA Phe.酵母苯丙氨酸转运核糖核酸二级和三级结构中质子交换率的核磁共振研究。
Nucleic Acids Res. 1977 Oct;4(10):3599-615. doi: 10.1093/nar/4.10.3599.
9
10
Fluorescent derivatives of yeast tRNAPhe.酵母苯丙氨酸转运核糖核酸的荧光衍生物
Eur J Biochem. 1979 Aug 1;98(2):465-75. doi: 10.1111/j.1432-1033.1979.tb13207.x.
引用本文的文献
1
Role of Wybutosine and Mg Ions in Modulating the Structure and Function of tRNA: A Molecular Dynamics Study.怀布托辛和镁离子在调节tRNA结构与功能中的作用：一项分子动力学研究
ACS Omega. 2019 Dec 2;4(25):21327-21339. doi: 10.1021/acsomega.9b02238. eCollection 2019 Dec 17.
2
Transfer-RNA, an early gene?转运核糖核酸，一种早期基因？
Naturwissenschaften. 1981 Jun;68(6):282-92. doi: 10.1007/BF01047470.
3
Selective binding of amino acid residues to tRNAPhe.氨基酸残基与苯丙氨酸转运RNA的选择性结合。
Nucleic Acids Res. 1984 Oct 11;12(19):7549-63. doi: 10.1093/nar/12.19.7549.
4
The conformation of the tRNAPhe anticodon loop monitored by fluorescence.通过荧光监测的苯丙氨酸转运RNA反密码子环的构象。
Nucleic Acids Res. 1984 Feb 24;12(4):2157-70. doi: 10.1093/nar/12.4.2157.
5
High resolution phosphorus NMR spectroscopy of transfer ribonucleic acids.转移核糖核酸的高分辨率磷核磁共振光谱学
Mol Cell Biochem. 1982 Jul 23;46(2):97-120. doi: 10.1007/BF00236777.
6
Predicting coding function from nucleotide sequence or survival of "fitness" of tRNA.从核苷酸序列预测编码功能或tRNA“适应性”的存活情况。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3539-43. doi: 10.1073/pnas.77.6.3539.
7
Mechanism of codon recognition by transfer RNA studied with oligonucleotides larger than triplets.用大于三联体的寡核苷酸研究转运RNA的密码子识别机制。
Nucleic Acids Res. 1985 May 24;13(10):3667-83. doi: 10.1093/nar/13.10.3667.
8
Conformational dynamics of the anticodon loop in yeast tRNAPhe as sensed by the fluorescence of wybutine.由怀丁荧光所感知的酵母苯丙氨酸转运核糖核酸反密码子环的构象动力学
Eur Biophys J. 1986;13(6):331-42. doi: 10.1007/BF00265669.
本文引用的文献
1
Thermodynamics and kinetics of the interaction of phenylalanine-specific tRNA from yeast with its cognate synthetase as studied by the flourescence of the Y-base.通过Y碱基荧光研究酵母苯丙氨酸特异性tRNA与其同源合成酶相互作用的热力学和动力学
FEBS Lett. 1973 Feb 15;30(1):6-10. doi: 10.1016/0014-5793(73)80606-4.
2
Conformation of the anticodon loop intRNA.tRNA中反密码子环的构象。
Nature. 1967 Aug 19;215(5103):817-21. doi: 10.1038/215817a0.
3
Molecular mechanics of translation: a reciprocating ratchet mechanism.翻译的分子机制：一种往复式棘轮机制。
Nature. 1970 May 30;226(5248):817-20. doi: 10.1038/226817a0.
4
Translocation of messenger RNA and "accommodation" of fMet-tRNA.信使核糖核酸的易位及甲酰甲硫氨酰转运核糖核酸的“容纳”
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1791-5. doi: 10.1073/pnas.68.8.1791.
5
Structure of yeast phenylalanine tRNA at 3 A resolution.酵母苯丙氨酸tRNA在3埃分辨率下的结构
Nature. 1974 Aug 16;250(467):546-51. doi: 10.1038/250546a0.
6
Spectroscopic properties of oligonucleotides excised from the anticodon region of phenylalanine tRNA from yeast.从酵母苯丙氨酸tRNA反密码子区域切除的寡核苷酸的光谱性质。
Biopolymers. 1973;12(1):27-43. doi: 10.1002/bip.1973.360120104.
7
Three-dimensional structure of yeast phenylalanine transfer RNA: folding of the polynucleotide chain.酵母苯丙氨酸转运核糖核酸的三维结构：多核苷酸链的折叠
Science. 1973 Jan 19;179(4070):285-8. doi: 10.1126/science.179.4070.285.
8
Tertiary structure of tRNAPhe (yeast): kinetics and electrostatic repulsion.酵母苯丙氨酸转运核糖核酸（tRNAPhe）的三级结构：动力学与静电排斥
Eur J Biochem. 1975 Jul 1;55(2):439-44. doi: 10.1111/j.1432-1033.1975.tb02180.x.
9
A speculation on the origin of protein synthesis.关于蛋白质合成起源的一种推测。
Orig Life. 1976 Dec;7(4):389-97. doi: 10.1007/BF00927934.
10
Codon-dependent rearrangement of the three-dimensional structure of phenylalanine tRNA, exposing the T-psi-C-G sequence for binding to the 50S ribosomal subunit.密码子依赖型苯丙氨酸tRNA三维结构重排，使T-ψ-C-G序列暴露以结合50S核糖体亚基。
Biochemistry. 1976 Jun 1;15(11):2484-90. doi: 10.1021/bi00656a035.
Zotero 插件