酵母苯丙氨酸转运核糖核酸二级和三级结构中质子交换率的核磁共振研究。
An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNA Phe.
作者信息
Johnston P D, Redfield A G
出版信息
Nucleic Acids Res. 1977 Oct;4(10):3599-615. doi: 10.1093/nar/4.10.3599.
Abstract
Solvent exchange rates of all the protons of yeast tRNAphe resonating in the lowfield NMR region (-11 to-15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNAphe are in the fast exchange limit with H2O relative to their intrinsic longitudinal relaxation processes. Most rates show very little temperature dependence; however, tertiary base pair protons are preferentially destabilized in the absence of Mg++ at higher temperatures. The measured exchange rates are between 2 and 125 sec-1 for a temperature range from 10 degrees C to 45 degrees C and MgCl2 concentrations between 0 and 15 mM.
摘要
通过饱和恢复长脉冲傅里叶变换核磁共振技术，测量了酵母苯丙氨酸转运核糖核酸（tRNAphe）在低场核磁共振区域（相对于DSS为-11至-15 ppm）共振的所有质子的溶剂交换率。相对于其固有的纵向弛豫过程，酵母tRNAphe中的所有这些质子与水都处于快速交换极限。大多数速率对温度的依赖性很小；然而，在较高温度且不存在Mg++的情况下，三级碱基对质子会优先失稳。在10℃至45℃的温度范围内以及MgCl2浓度在0至15 mM之间时，测得的交换率在2至125秒-1之间。
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