信使核糖核酸的易位及甲酰甲硫氨酰转运核糖核酸的“容纳”
Translocation of messenger RNA and "accommodation" of fMet-tRNA.
作者信息
Thach S S, Thach R E
出版信息
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1791-5. doi: 10.1073/pnas.68.8.1791.
Abstract
Messenger RNA is moved a distance of approximately three nucleotides in the 5' direction relative to the ribosome during the translocation of peptidyl-tRNA from the A to the P site. This movement is catalyzed by G factor and is dependent on the hydrolysis of GTP. In contrast, mRNA is not moved during the f(2)-catalyzed hydrolysis of GTP that is involved in the activation of ribosome-bound fMet-tRNA. This second type of GTP-dependent reaction has been named "Accommodation".
摘要
在肽基 - tRNA从A位点转移至P位点的移位过程中，信使核糖核酸（mRNA）相对于核糖体在5'方向上移动约三个核苷酸的距离。这种移动由G因子催化，并依赖于鸟苷三磷酸（GTP）的水解。相比之下，在参与核糖体结合的甲酰甲硫氨酸 - tRNA（fMet - tRNA）激活的GTP的f(2)催化水解过程中，mRNA并不移动。这种第二种类型的GTP依赖性反应被命名为“容纳”。
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