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2
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RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
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Replacement of Y base, dihydrouracil, and 7-methylguanine in tRNA by artificial odd bases.通过人工奇碱基替换tRNA中的Y碱基、二氢尿嘧啶和7-甲基鸟嘌呤。
FEBS Lett. 1971 Nov 1;18(2):214-218. doi: 10.1016/0014-5793(71)80447-7.
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New fluorescent hydrazide reagents for the oxidized 3'-terminus of RNA.用于RNA氧化3'-末端的新型荧光酰肼试剂。
Nucleic Acids Res. 1974 Jun;1(6):767-86. doi: 10.1093/nar/1.6.767.
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A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid.苯丙氨酸转移核糖核酸反密码子旁的一种特定修饰。
Eur J Biochem. 1968 Sep 24;5(4):546-55. doi: 10.1111/j.1432-1033.1968.tb00404.x.
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Studies on polynucleotides. LXXX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with ribonuclease T1.多核苷酸的研究。八十。酵母苯丙氨酸转移核糖核酸：核糖核酸酶T1降解产物
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Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.在聚氯三氟乙烯支持的反相色谱柱上改进转运RNA的分离。
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7
Structure of the fluorescent Y base from yeast phenylalanine transfer ribonucleic acid.来自酵母苯丙氨酸转移核糖核酸的荧光Y碱基的结构。
J Am Chem Soc. 1970 Dec 30;92(26):7617-9. doi: 10.1021/ja00729a035.
8
Luminescence and binding studies on tRNA-Phe.对苯丙氨酸转运核糖核酸（tRNA-Phe）的发光及结合研究。
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9
Further studies on amino acid acceptance and physical properties of tRNA-phe-yeast.酵母苯丙氨酸tRNA的氨基酸接纳及物理性质的进一步研究
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10
L-phenylalanyl-tRNA synthetase of Escherichia coli K-10. A reinvestigation of molecular weight and subunit structure.大肠杆菌K-10的L-苯丙氨酰-tRNA合成酶。分子量和亚基结构的重新研究。
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用肼取代怀丁碱及其对酵母苯丙氨酸转运核糖核酸活性构象的影响。

Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe.

作者信息

Schleich H G, Wintermeyer W, Zachau H G

出版信息

Nucleic Acids Res. 1978 May;5(5):1701-13. doi: 10.1093/nar/5.5.1701.

DOI:10.1093/nar/5.5.1701
PMID:351568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342114/
Abstract

The highly modified base wybutine (YWye) next to the anticodon of yeast tRNAPhe has been replaced by different hydrazine derivatives. The effect of the replacement on the activity of the tRNA has been studied in the heterologous aminoacylation with synthetase from E. coli and in the poly(U) directed binding to ribosomes from both yeast and E. coli. It was found that starting from tRNA-PheYWye the activity increased with increasing size, aromaticity, and stacking tendency of the substituent replacing YWye. It is concluded that YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.

摘要

酵母苯丙氨酸转运核糖核酸（tRNAPhe）反密码子旁高度修饰的碱基怀丁（YWye）已被不同的肼衍生物所取代。通过与来自大肠杆菌的合成酶进行异源氨酰化反应以及在聚（U）引导下与来自酵母和大肠杆菌的核糖体结合，研究了这种取代对tRNA活性的影响。结果发现，从tRNA-PheYWye开始，随着取代YWye的取代基尺寸增大、芳香性增强和堆积倾向增加，活性也随之提高。结论是，YWye凭借其芳香体系的大小及其堆积特性，特别适合稳定tRNAPhe的天然构象。