用肼取代怀丁碱及其对酵母苯丙氨酸转运核糖核酸活性构象的影响。
Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe.
作者信息
Schleich H G, Wintermeyer W, Zachau H G
出版信息
Nucleic Acids Res. 1978 May;5(5):1701-13. doi: 10.1093/nar/5.5.1701.
Abstract
The highly modified base wybutine (YWye) next to the anticodon of yeast tRNAPhe has been replaced by different hydrazine derivatives. The effect of the replacement on the activity of the tRNA has been studied in the heterologous aminoacylation with synthetase from E. coli and in the poly(U) directed binding to ribosomes from both yeast and E. coli. It was found that starting from tRNA-PheYWye the activity increased with increasing size, aromaticity, and stacking tendency of the substituent replacing YWye. It is concluded that YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.
摘要
酵母苯丙氨酸转运核糖核酸（tRNAPhe）反密码子旁高度修饰的碱基怀丁（YWye）已被不同的肼衍生物所取代。通过与来自大肠杆菌的合成酶进行异源氨酰化反应以及在聚（U）引导下与来自酵母和大肠杆菌的核糖体结合，研究了这种取代对tRNA活性的影响。结果发现，从tRNA-PheYWye开始，随着取代YWye的取代基尺寸增大、芳香性增强和堆积倾向增加，活性也随之提高。结论是，YWye凭借其芳香体系的大小及其堆积特性，特别适合稳定tRNAPhe的天然构象。
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