亚马逊东部森林碎片化地区钩端螺旋体的检测：一种独特的健康研究方法。
Detection of Leptospira in a forest fragmentation area in eastern Amazon: A unique health approach.
作者信息
de Souza Rocha Katarine, da Rocha Albuquerque Mírian, da Silva Brito Jacqueline, Pimenta Gabriela Castanheira, Dos Reis Thalita Amaral, Xavier Diego Arruda, Guimarães de Moraes Carla Cristina
机构信息
Laboratório de Zoonoses e Saúde Pública, Instituto de Medicina Veterinária, Universidade Federal do Pará, Brazil.
Programa de Capacitação Institucional, Coordenação Ciência da Terra e Ecologia, Museu Paraense Emílio Goeldi, Campus de Pesquisa, Brazil.
出版信息
Comp Immunol Microbiol Infect Dis. 2022 Jan 31;82:101757. doi: 10.1016/j.cimid.2022.101757.
The objective of this study was to detect the presence of anti-Leptospira spp. in samples of humans and domestic animals of forest fragmentation areas in the Brazilian Amazon. A total of 208 samples from dogs, cats, and humans were collected during two expeditions within the same fragment. Detection of anti-Leptospira spp. antibodies was performed by the Microscopic Agglutination Test (MAT) using live antigens from 19 Leptospira spp. serogroups and DNA detection of Leptospira 16S rRNA gene was done using Polymerase Chain Reaction. After the analysis, we observed 24.03% (50/208) of reactive serum samples, where the most prevalent serogroups circulating in the human and animal population were Canicola, Djasiman, Cynopteri, and Serjoe. Leptospira DNA was detected only in the samples of 13 dogs, which demonstrated 100% identity with L. interrogans DNA deposited in the GenBank. We concluded that there is a circulation of Leptospira spp. in the studied fragment.
本研究的目的是检测巴西亚马逊森林破碎化地区人类和家畜样本中抗钩端螺旋体属的存在情况。在同一区域内的两次考察期间，共采集了来自狗、猫和人类的208份样本。采用显微镜凝集试验（MAT），使用19个钩端螺旋体属血清群的活抗原检测抗钩端螺旋体属抗体，并采用聚合酶链反应进行钩端螺旋体16S rRNA基因的DNA检测。分析后，我们观察到24.03%（50/208）的血清样本呈反应性，其中在人类和动物群体中传播最普遍的血清群是犬钩端螺旋体、爪哇群、犬蝠群和赛尔群。仅在13只狗的样本中检测到钩端螺旋体DNA，其与GenBank中存放的问号钩端螺旋体DNA具有100%的同一性。我们得出结论，在所研究的区域内存在钩端螺旋体属的传播。
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