de Lisbôa Padilha Mayla, Ricardo Bezerra de Melo Lídio, Henrique Limeira Clécio, Maria de Andrade Magalhães Nathália, Luana Cristiny Rodrigues Silva Maria, Rodrigues Soares Rafael, José Alves Clebert, Silvano Dos Santos Higino Severino, de Sousa Américo Batista Santos Carolina, Santos de Azevedo Sérgio
Post-graduate Program of Science and Animal Health, Academic Unit of Veterinary Medicine, Federal University of Campina Grande, Patos, Paraíba State, Brazil.
Post-graduate Program of Science and Animal Health, Academic Unit of Veterinary Medicine, Federal University of Campina Grande, Patos, Paraíba State, Brazil.
Rev Argent Microbiol. 2025 Jul-Sep;57(3):265-274. doi: 10.1016/j.ram.2025.03.001. Epub 2025 Apr 7.
The Caatinga biome occurs only in Brazil; however, there is no survey on leptospirosis using direct and indirect diagnostic tests in this region with goats maintained in field conditions. We conducted a prospective survey with paired sampling to evaluate the importance of carrier goats in the maintenance of disease. Based on sampling calculation, 60 goats (30 confined and 30 extensively reared) were randomly selected and monitored for three months during the rainy season with paired monthly biological sample collections. The animals underwent urine and vaginal fluid collection for microbiological and molecular diagnosis, and serum samples for serological diagnosis using the microscopic agglutination test (MAT). Overall, 45 (75%) animals were positive in at least one diagnostic method. Anti-Leptospira spp. antibodies were detected in all three sampling moments in 43 (71.7%) animals, antibody titers varied from 25 to 800, and most frequent serogroups were Australis in the 1st and 2nd blood collections (66.7% and 40.7%, respectively) and Cynopteri in the 3rd collection (52.6%). None of the animals tested positive in the microbiological diagnosis or the vaginal fluid PCR; however, five (16.7%) animals were positive in the urine PCR only in the confined group in the first collection. Two DNA urine samples were sequenced, demonstrating 99% similarity with Leptospira interrogans. Different diagnostic techniques for leptospirosis in goats raised under Caatinga biome field conditions are suggested, as well as further studies over a longer period with monthly collections to better understand the prevalence of Leptospira spp. and its variation over time.
卡廷加生物群落仅存在于巴西；然而，在该地区，尚未有针对野外饲养山羊的钩端螺旋体病进行直接和间接诊断检测的调查。我们进行了一项前瞻性配对抽样调查，以评估携带病菌的山羊在疾病传播中的重要性。根据抽样计算，随机选取了60只山羊（30只圈养和30只粗放饲养），在雨季进行为期三个月的监测，每月成对采集生物样本。对动物进行尿液和阴道分泌物采集，用于微生物学和分子诊断，并采集血清样本，采用显微镜凝集试验（MAT）进行血清学诊断。总体而言，45只（75%）动物至少有一种诊断方法呈阳性。在43只（71.7%）动物的所有三个采样时刻均检测到抗钩端螺旋体属抗体，抗体效价从25到800不等，最常见的血清群在第一次和第二次采血时为澳洲群（分别为66.7%和40.7%），第三次采血时为犬群（52.6%）。在微生物学诊断或阴道分泌物PCR检测中，没有动物呈阳性；然而，仅在第一次采集时，圈养组中有5只（16.7%）动物尿液PCR呈阳性。对两份尿液DNA样本进行了测序，结果显示与问号钩端螺旋体的相似度为99%。建议对卡廷加生物群落野外条件下饲养的山羊采用不同的钩端螺旋体病诊断技术，并进行更长时间、每月采集样本的进一步研究，以更好地了解钩端螺旋体属的流行情况及其随时间的变化。
