Institute of Pathology, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia.
Cells. 2022 Jan 18;11(3):314. doi: 10.3390/cells11030314.
Fibrosis is an important feature of inflammatory bowel diseases (IBD), but its pathogenesis is incompletely understood. Our aim was to identify genes important for fibrosis in IBD by comparison with kidney and liver fibrosis. First, we performed bioinformatics analysis of Gene Expression Omnibus datasets of liver and kidney fibrosis and identified and as potentially important genes with altered expression in fibrosis. We then performed qPCR analysis of the selected genes' expression on samples of fibrotic kidney, liver, Crohn's disease (CD) with and without fibrosis and ulcerative colitis (UC), in comparison to corresponding normal tissue. We found significantly altered expression in fibrosis for all selected genes. A significant difference for some genes was observed in CD with fibrosis in comparison to CD without fibrosis and UC. We conclude that similar changes in the expression of selected genes in liver, kidney fibrosis and IBD provide further evidence that fibrosis in IBD might share common mechanisms with other organs, supporting the hypothesis that fibrosis is the common pathway in diseases of various organs. Some genes were already active in IBD with inflammation without fibrosis, suggesting the early activation of profibrotic pathways or overlapping function in fibrosis and inflammation.
纤维化是炎症性肠病 (IBD) 的一个重要特征，但其发病机制尚不完全清楚。我们的目的是通过与肝和肾纤维化进行比较，鉴定与 IBD 纤维化相关的重要基因。首先，我们对肝和肾纤维化的基因表达综合数据库 (Gene Expression Omnibus datasets) 进行了生物信息学分析，确定 和 为纤维化中表达改变的潜在重要基因。然后，我们对纤维化的肾脏、肝脏、有和没有纤维化的克罗恩病 (CD) 以及溃疡性结肠炎 (UC) 的样本进行了所选基因表达的 qPCR 分析，并与相应的正常组织进行了比较。我们发现所有选定基因在纤维化中表达均发生显著改变。在有纤维化的 CD 与无纤维化的 CD 和 UC 之间，一些基因的表达存在显著差异。我们得出结论，在肝、肾纤维化和 IBD 中选定基因表达的相似变化进一步证明了 IBD 中的纤维化可能与其他器官具有共同的机制，支持纤维化是各种器官疾病的共同途径的假说。一些基因在没有纤维化的炎症性肠病中就已经活跃，这表明纤维化途径的早期激活或纤维化和炎症之间的重叠功能。
