Department of Clinical Biochemistry, Addenbrookes Biomedical Campus, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK.
Cells. 2022 Jan 20;11(3):347. doi: 10.3390/cells11030347.
Any given cell type has an associated "normal" nuclear morphology, which is important to maintain proper cellular functioning and safeguard genomic integrity. Deviations from this can be indicative of diseases such as cancer or premature aging syndrome. To accurately assess nuclear abnormalities, it is important to use quantitative measures of nuclear morphology. Here, we give an overview of several nuclear abnormalities, including micronuclei, nuclear envelope invaginations, blebs and ruptures, and review the current methods used for image-based quantification of these abnormalities. We discuss several parameters that can be used to quantify nuclear shape and compare their outputs using example images. In addition, we present new pipelines for quantitative analysis of nuclear blebs and invaginations. Quantitative analyses of nuclear aberrations and shape will be important in a wide range of applications, from assessments of cancer cell anomalies to studies of nucleus deformability under mechanical or other types of stress.
任何给定的细胞类型都有其相关的“正常”核形态，这对于维持正常的细胞功能和保护基因组完整性非常重要。偏离这种形态可能表明存在癌症或早衰综合征等疾病。为了准确评估核异常，使用核形态的定量测量非常重要。在这里，我们概述了几种核异常，包括微核、核膜内陷、泡和破裂，并回顾了目前用于这些异常的基于图像的定量方法。我们讨论了可用于量化核形状的几个参数，并使用示例图像比较它们的输出。此外，我们还提出了用于定量分析核泡和内陷的新管道。核异常和形状的定量分析将在广泛的应用中非常重要，从评估癌细胞异常到研究机械或其他类型的应激下核的变形性。
