Bourgès Anaïs C, Garre Massimiliano, Wu Dan, O'Shea Donal F
Department of Chemistry, RCSI, University of Medicine and Health Sciences, 123 St Stephen's Green, D02 YN77 Dublin, Ireland.
Membranes (Basel). 2025 Jan 1;15(1):9. doi: 10.3390/membranes15010009.
The endoplasmic reticulum and the internal nuclear compartments are intrinsically connected through the nuclear membrane, pores and lamina. High resolution imaging of each of these cellular features concurrently remains a significant challenge. To that end we have developed a new molecular nuclear membrane-endoplasmic reticulum (NM-ER) staining fluorophore with emission maxima at 650 nm. NM-ER is compatible with fixed and live cell imaging and stimulated emission depletion microscopy (STED) showing significant improvement in resolution when compared to comparable confocal laser scanning microscopy. The imaging versatility of NM-ER was illustrated through its compatible use with other fluorophores for co-imaging with DNA, nuclear pores and lamina allowing cellular abnormalities to be identified. NM-ER alone, or in use with other nuclear region labels could be an important tool for the investigation of nuclear transport and associated cellular processes.
内质网与细胞核内部隔室通过核膜、核孔和核纤层存在内在联系。同时对这些细胞特征中的每一个进行高分辨率成像仍然是一项重大挑战。为此，我们开发了一种新的分子核膜 - 内质网（NM - ER）染色荧光团，其最大发射波长为650 nm。NM - ER适用于固定细胞和活细胞成像以及受激发射损耗显微镜（STED），与可比的共聚焦激光扫描显微镜相比，分辨率有显著提高。通过将NM - ER与其他荧光团兼容用于与DNA、核孔和核纤层的共成像，展示了其成像多功能性，从而能够识别细胞异常情况。单独使用NM - ER或与其他核区域标记物一起使用，可能是研究核运输及相关细胞过程的重要工具。
