Maddala Rupalatha, Ho Leona T Y, Karnam Shruthi, Navarro Iris, Osterwald Anja, Stinnett Sandra S, Ullmer Christoph, Vann Robin R, Challa Pratap, Rao Ponugoti V
Department of Ophthalmology, Duke University School of Medicine, Durham, NC 27710, USA.
Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., 4070 Basel, Switzerland.
J Clin Med. 2022 Jan 29;11(3):744. doi: 10.3390/jcm11030744.
Dysregulated levels of growth/differentiation factor-15 (GDF15), a divergent member of the transforming growth factor-beta super family, have been found to be associated with the pathology of various diseases. In this study, we evaluated the levels of GDF15 in aqueous humor (AH) and serum samples derived from primary open-angle glaucoma (POAG) and age- and gender-matched non-glaucoma (cataract) patients to assess the plausible association between GDF15 and POAG. GDF15 levels were determined using an enzyme-linked immunosorbent assay, and data analysis was performed using the Wilcoxon rank sum test, or the Kruskal-Wallis test and linear regression. GDF15 levels in the AH ( = 105) of POAG patients were significantly elevated (by 7.4-fold) compared to cataract patients ( = 117). Serum samples obtained from a subgroup of POAG patients ( = 41) also showed a significant increase in GDF15 levels (by 50%) compared to cataract patients. GDF15 levels were elevated in male, female, African American, and Caucasian POAG patients. This study reveals a significant and marked elevation of GDF15 levels in the AH of POAG patients compared to non-glaucoma cataract control patients. Although serum GDF15 levels were also elevated in POAG patients, the magnitude of difference was much smaller relative to that found in the AH.
生长/分化因子-15（GDF15）是转化生长因子-β超家族的一个不同成员，其水平失调已被发现与多种疾病的病理过程相关。在本研究中，我们评估了原发性开角型青光眼（POAG）患者以及年龄和性别匹配的非青光眼（白内障）患者房水（AH）和血清样本中GDF15的水平，以评估GDF15与POAG之间可能存在的关联。使用酶联免疫吸附测定法测定GDF15水平，并使用Wilcoxon秩和检验、Kruskal-Wallis检验和线性回归进行数据分析。与白内障患者（n = 117）相比，POAG患者房水（n = 105）中的GDF15水平显著升高（7.4倍）。从一组POAG患者（n = 41）获得的血清样本与白内障患者相比，GDF15水平也显著升高（50%）。男性、女性、非裔美国人和白种人POAG患者的GDF15水平均升高。本研究揭示，与非青光眼白内障对照患者相比，POAG患者房水中GDF15水平显著且明显升高。虽然POAG患者血清GDF15水平也升高，但与房水相比，差异幅度要小得多。
