Hagbani Turki Al, Yadav Hemant, Moin Afrasim, Lila Amr Selim Abu, Mehmood Khalid, Alshammari Farhan, Khan Salman, Khafagy El-Sayed, Hussain Talib, Rizvi Syed Mohd Danish, Abdallah Marwa H
Department of Pharmaceutics, College of Pharmacy, University of Ha'il, Ha'il 81442, Saudi Arabia.
Department of Pharmaceutics, RAK College of Pharmaceutical Sciences, RAK Medical & Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates.
Materials (Basel). 2022 Jan 31;15(3):1108. doi: 10.3390/ma15031108.
The remarkable rise of antibiotic resistance among pathogenic bacteria poses a significant threat to human health. Nanoparticles (NPs) have recently emerged as novel strategies for conquering fatal bacterial diseases. Furthermore, antibiotic-functionalized metallic NPs represent a viable nano-platform for combating bacterial resistance. In this study, we present the use of vancomycin-functionalized gold nanoparticles (V-GNPs) to battle pathogenic bacterial strains. A facile one-pot method was adopted to synthesize vancomycin-loaded GNPs in which the reducing properties of vancomycin were exploited to produce V-GNPs from gold ions. UV-Visible spectroscopy verified the production of V-GNPs via the existence of a surface plasmon resonance peak at 524 nm, whereas transmission electron microscopy depicted a size of ~24 nm. Further, dynamic light scattering (DLS) estimated the hydrodynamic diameter as 77 nm. The stability of V-GNPs was investigated using zeta-potential measurements, and the zeta potential of V-GNPs was found to be -18 mV. Fourier transform infrared spectroscopy confirmed the efficient loading of vancomycin onto GNP surfaces; however, the loading efficiency of vancomycin onto V-GNPs was 86.2%. Finally, in vitro antibacterial studies revealed that V-GNPs were much more effective, even at lower concentrations, than pure vancomycin. The observed antibacterial activities of V-GNPs were 1.4-, 1.6-, 1.8-, and 1.6-fold higher against Gram-negative , , and and Gram-positive , respectively, compared to pure vancomycin. Collectively, V-GNPs represented a more viable alternative to pure vancomycin, even at a lower antibiotic dose, in conquering pathogenic bacteria.
致病细菌中抗生素耐药性的显著上升对人类健康构成了重大威胁。纳米颗粒（NPs）最近已成为攻克致命细菌性疾病的新策略。此外，抗生素功能化金属纳米颗粒是对抗细菌耐药性的可行纳米平台。在本研究中，我们展示了使用万古霉素功能化金纳米颗粒（V-GNPs）对抗致病细菌菌株。采用一种简便的一锅法合成负载万古霉素的金纳米颗粒，利用万古霉素的还原特性从金离子中制备V-GNPs。紫外可见光谱通过在524nm处存在表面等离子体共振峰验证了V-GNPs的产生，而透射电子显微镜显示其尺寸约为24nm。此外，动态光散射（DLS）估计其流体动力学直径为77nm。使用zeta电位测量研究了V-GNPs的稳定性，发现V-GNPs的zeta电位为-18mV。傅里叶变换红外光谱证实万古霉素有效地负载到了金纳米颗粒表面；然而，万古霉素在V-GNPs上的负载效率为86.2%。最后，体外抗菌研究表明，即使在较低浓度下，V-GNPs也比纯万古霉素更有效。与纯万古霉素相比，观察到V-GNPs对革兰氏阴性菌、、和革兰氏阳性菌的抗菌活性分别高1.4倍、1.6倍、1.8倍和1.6倍。总体而言，在攻克致病细菌方面，即使使用较低剂量的抗生素，V-GNPs也是比纯万古霉素更可行的替代品。
