Mesulam M M, Mufson E J, Wainer B H
Brain Res. 1986 Mar 5;367(1-2):301-8. doi: 10.1016/0006-8993(86)91607-0.
Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents.
投射到新皮层的基底核神经元中有96%含有胆碱乙酰转移酶。基底核胆碱能成分（Ch4）的这些投射在拓扑结构上是有组织的，因此每个皮层区域的大部分胆碱能输入都来自不同的Ch4区域。这些区域的三维重建显示出一种复杂结构的存在。这些实验中使用的一种稳定程序保留了四甲基联苯胺法用于辣根过氧化物酶的所有优点，同时消除了反应产物对高pH值和脱水剂的敏感性。
