Rye D B, Wainer B H, Mesulam M M, Mufson E J, Saper C B
Neuroscience. 1984 Nov;13(3):627-43. doi: 10.1016/0306-4522(84)90083-6.
The neurochemical identity of ascending putative cholinergic pathways from the rat basal forebrain was investigated employing a method for simultaneously visualizing choline acetyltransferase immunoreactivity and retrogradely transported horseradish peroxidase-conjugated wheatgerm agglutinin. This histochemical procedure revealed three distinct populations of neurons: (1) cells which stained only for choline acetyltransferase immunoreactivity; (2) cells which stained only for retrograde tracer and (3) cells which stained simultaneously for choline acetyltransferase immunoreactivity and retrograde tracer. The results demonstrated that this projection is topographically organized and consists of both cholinergic and noncholinergic components. The relative contribution of each component varied with the telencephalic target area as follows: the olfactory bulb receives a projection from cells of the horizontal limb nucleus, 10-20% of which are cholinergic (Ch3); the hippocampal formation receives afferents from cells of the medial septal and vertical limb nuclei, 35-45% of which are cholinergic (Ch1 and Ch2); and the cortical mantle receives afferents primarily from cells within the substantia innominata-nucleus basalis complex, 80-90% of which are cholinergic (Ch4). The topographical organization of Ch4 projections is not as completely differentiated as we have previously observed in the primate.
采用同时可视化胆碱乙酰转移酶免疫反应性和逆行转运的辣根过氧化物酶结合麦胚凝集素的方法，对大鼠基底前脑上行假定胆碱能通路的神经化学特性进行了研究。这种组织化学方法揭示了三种不同的神经元群体：（1）仅对胆碱乙酰转移酶免疫反应性染色的细胞；（2）仅对逆行示踪剂染色的细胞；（3）同时对胆碱乙酰转移酶免疫反应性和逆行示踪剂染色的细胞。结果表明，该投射在地形上是有组织的，由胆碱能和非胆碱能成分组成。每个成分的相对贡献随端脑靶区的不同而变化，如下所示：嗅球接受来自水平肢核细胞的投射，其中10%-20%是胆碱能的（Ch3）；海马结构接受来自内侧隔核和垂直肢核细胞的传入纤维，其中35%-45%是胆碱能的（Ch1和Ch2）；皮质接受主要来自无名质-基底核复合体中细胞的传入纤维，其中80%-90%是胆碱能的（Ch4）。Ch4投射的地形组织不像我们之前在灵长类动物中观察到的那样完全分化。
