Department of Physiology, Kangwon National University School of Medicine, Chuncheon 24341, Korea.
Department of Anesthesiology and Pain Medicine, Busan Paik Hospital, Inje University, Busan 47392, Korea.
Int J Mol Sci. 2022 Jan 22;23(3):1228. doi: 10.3390/ijms23031228.
Inflammasomes are a group of intracellular multiprotein platforms that play important roles in immune systems. Benzyl isothiocyanate (BITC) is a constituent of cruciferous plants and has been confirmed to exhibit various biological activities. The modulatory effects of BITC on inflammasome-mediated interleukin (IL)-1β expression and its regulatory mechanisms in () LPS/ATP-stimulated THP-1 cells was investigated. Monocytic THP-1 cells were treated with phorbol myristate acetate (PMA) to induce differentiation into macrophages. Enzyme-linked immunosorbent assays (ELISA) were performed to measure the levels of IL-1β produced in LPS/ATP-exposed THP-1 cells. Western blotting was performed to examine the BITC modulatory mechanisms in inflammasome-mediated signaling pathways. BITC inhibited IL-1β production in LPS/ATP-induced THP-1 cells. BITC also inhibited activation of leucine-rich repeat protein-3 (NLRP3) and caspase-1 in LPS/ATP-induced THP-1 cells. Furthermore, we show that mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) activation in LPS was attenuated by BITC. These BITC-mediated modulatory effects on IL-1β production may have therapeutic potential for inflammasome-mediated disorders such as a nasal polyp.
炎性小体是一组在免疫系统中发挥重要作用的细胞内多蛋白平台。苯乙基异硫氰酸酯（BITC）是十字花科植物的成分之一，已被证实具有多种生物活性。本研究旨在探讨 BITC 对 LPS/ATP 刺激的 THP-1 细胞中介炎性小体介导的白细胞介素（IL）-1β表达的调节作用及其调节机制。用佛波醇肉豆蔻酸酯（PMA）处理单核细胞 THP-1 细胞以诱导其分化为巨噬细胞。酶联免疫吸附试验（ELISA）用于测量 LPS/ATP 暴露的 THP-1 细胞中产生的 IL-1β水平。通过 Western blot 检测 BITC 在炎性小体介导的信号通路中的调节机制。BITC 抑制 LPS/ATP 诱导的 THP-1 细胞中 IL-1β的产生。BITC 还抑制了 LPS/ATP 诱导的 THP-1 细胞中富含亮氨酸重复蛋白-3（NLRP3）和半胱天冬酶-1 的激活。此外，我们还表明，BITC 减弱了 LPS 中丝裂原活化蛋白激酶（MAPK）和核因子-κB（NF-κB）的激活。这些 BITC 介导的对 IL-1β产生的调节作用可能对炎性小体介导的疾病（如鼻息肉）具有治疗潜力。
