Department of Pathophysiology, Institute of Medical Sciences, University of Rzeszow, 35-959 Rzeszów, Poland.
Department of Animal Physiology and Toxicology, Pedagogical University in Cracow, 30-059 Cracow, Poland.
Molecules. 2022 Jan 31;27(3):965. doi: 10.3390/molecules27030965.
Our research aimed to show acrylamide's influence on inflammatory processes, the oxidative stress it causes in the cholinergic system, and the possibility of reducing inflammation via supplementation with α-tocopherol. For this purpose, an in ovo model was used where the embryos were exposed to acrylamide, α-tocopherol and a cocktail of these substances. After 48 h of exposure, we collected brain samples and performed biochemical assays to examine the effect of the chosen substances on oxidative stress (malondialdehyde-MDA and reduced glutathione-GSH) and acetylcholinesterase activity (AChE). The results showed that acrylamide decreased AChE activity in the examined brain samples by about 25% in comparison to the control group, and this effect was decreased by administering α-tocopherol. The concentration of malondialdehyde significantly increased in the group given acrylamide, while, in the group with α-tocopherol, the observed concentration was lower in comparison to the control group. Moreover, a decrease in glutathione concentration was observed after the administration of acrylamide; however, the protective effect of α-tocopherol was only slightly visible in this case. In conclusion, α-tocopherol minimizes the harmful effects of acrylamide on AchE, and it can minimize the concentration of MDA.
我们的研究旨在展示丙烯酰胺对炎症过程的影响、它在胆碱能系统中引起的氧化应激，以及通过补充α-生育酚减少炎症的可能性。为此，我们使用了一种鸡胚模型，其中胚胎暴露于丙烯酰胺、α-生育酚和这些物质的混合物中。暴露 48 小时后，我们收集了脑组织样本并进行了生化分析，以检查所选物质对氧化应激（丙二醛-MDA 和还原型谷胱甘肽-GSH）和乙酰胆碱酯酶活性（AChE）的影响。结果表明，与对照组相比，丙烯酰胺使被检查的脑组织样本中的 AChE 活性降低了约 25%，而α-生育酚的给予则降低了这种作用。在给予丙烯酰胺的组中，丙二醛的浓度显著增加，而在给予α-生育酚的组中，与对照组相比，观察到的浓度较低。此外，在给予丙烯酰胺后观察到谷胱甘肽浓度降低；然而，在这种情况下，α-生育酚的保护作用仅略微可见。总之，α-生育酚可将丙烯酰胺对 AchE 的有害影响降至最低，并可将 MDA 的浓度降至最低。
