Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan P.O. Box 114, Saudi Arabia.
Molecules. 2022 Feb 1;27(3):971. doi: 10.3390/molecules27030971.
Mahanimbine (MN) is a carbazole alkaloid present in the leaves of which is an integral part of medicinal and culinary practices in Asia. In the present study, the anticancer, apoptotic and anti-invasive potential of MN has been delineated in vitro. Apoptosis cells determination was carried out utilizing the acridine orange/propidium iodide double fluorescence test. During treatment, caspase-3/7,-8, and-9 enzymes and mitochondrial membrane potentials (Δψm) were evaluated. Anti-invasive properties were tested utilizing a wound-healing scratch test. Protein and gene expression studies were used to measure Bax, Bcl2, MMP-2, and -9 levels. The results show that MN could induce apoptosis in MCF-7 cells at 14 µM concentration IC. MN-induced mitochondria-mediated apoptosis, with loss in Δψm, regulation of Bcl2/Bax, and accumulation of ROS ( ≤ 0.05). Caspase-3/7 and -9 enzyme activity were detected in MCF-7 cells after 24 and 48 h of treatment with MN. The anti-invasive property of MN was shown by inhibition of wound healing at the dose-dependent level and significantly suppressed mRNA and protein expression on MMP-2 and -9 in MCF-7 cells treated with a sub-cytotoxic dose of MN. The overall results indicate MN is a potential therapeutic compound against breast cancer as an apoptosis inducer and anti-invasive agent.
马汉宁（MN）是一种存在于 的咔唑生物碱， 是亚洲医学和烹饪实践不可或缺的一部分。在本研究中，MN 的抗癌、凋亡和抗侵袭潜力已在体外进行了描述。利用吖啶橙/碘化丙啶双重荧光试验进行凋亡细胞测定。在治疗过程中，评估了 caspase-3/7、-8 和-9 酶和线粒体膜电位（Δψm）。利用划痕愈合试验测试抗侵袭特性。蛋白质和基因表达研究用于测量 Bax、Bcl2、MMP-2 和 -9 水平。结果表明，MN 可在 14 µM 浓度 IC 下诱导 MCF-7 细胞凋亡。MN 诱导的线粒体介导的凋亡，伴随着 Δψm 的丧失、Bcl2/Bax 的调节和 ROS 的积累（≤0.05）。MN 处理 MCF-7 细胞 24 和 48 小时后，检测到 caspase-3/7 和 -9 酶活性。MN 的抗侵袭特性表现为在剂量依赖性水平上抑制伤口愈合，并显著抑制 MN 亚细胞毒性剂量处理的 MCF-7 细胞中 MMP-2 和 -9 的 mRNA 和蛋白表达。总体结果表明，MN 作为凋亡诱导剂和抗侵袭剂，是一种有潜力的治疗乳腺癌的化合物。
