Department of Pharmacy, Southeast University, Dhaka 1212, Bangladesh.
Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.
Molecules. 2022 Feb 3;27(3):1037. doi: 10.3390/molecules27031037.
Neurotoxicity is a serious health problem of patients chronically exposed to arsenic. There is no specific treatment of this problem. Oxidative stress has been implicated in the pathological process of neurotoxicity. Polyphenolics have proven antioxidant activity, thereby offering protection against oxidative stress. In this study, we have isolated the polyphenolics from and investigated its effect against arsenic-induced neurotoxicity and oxidative stress in mice. polyphenolics prepared from column chromatography of the crude methanol extract using diaion resin contained a phenolic content of 452.185 ± 7.879 mg gallic acid equivalent/gm of sample and flavonoid content of 200.075 ± 0.755 mg catechin equivalent/gm of sample. The polyphenolics exhibited potent antioxidant activity with respect to free radical scavenging ability, total antioxidant activity and inhibition of lipid peroxidation. Administration of arsenic in mice showed a reduction of acetylcholinesterase activity in the brain which was counteracted by polyphenolics. Similarly, elevation of lipid peroxidation and depletion of glutathione in the brain of mice was effectively restored to normal level by polyphenolics. Gallic acid methyl ester, catechin and catechin-7-gallate were identified in the polyphenolics as the major active compounds. These results suggest that polyphenolics due to its strong antioxidant potential might be effective in the management of arsenic induced neurotoxicity.
神经毒性是慢性砷暴露患者的一个严重健康问题。目前尚无针对该问题的特定治疗方法。氧化应激已被牵涉到神经毒性的病理过程中。多酚已被证明具有抗氧化活性，从而提供了针对氧化应激的保护。在这项研究中，我们从 中分离出多酚，并研究了其对小鼠砷诱导的神经毒性和氧化应激的影响。从粗甲醇提取物通过 Diaion 树脂柱层析制备的多酚，其没食子酸当量的酚含量为 452.185 ± 7.879mg/g，儿茶素当量的黄酮含量为 200.075 ± 0.755mg/g。多酚对自由基清除能力、总抗氧化活性和抑制脂质过氧化具有很强的抗氧化活性。给小鼠注射砷会降低大脑中的乙酰胆碱酯酶活性，而 多酚可以对抗这种降低。同样， 多酚可以有效地将小鼠大脑中的脂质过氧化和谷胱甘肽耗竭恢复到正常水平。多酚中鉴定出的主要活性化合物为没食子酸甲酯、儿茶素和儿茶素-7-没食子酸酯。这些结果表明，由于其强大的抗氧化潜力， 多酚可能对管理砷诱导的神经毒性有效。
