Faculty of Engineering Information and Systems, University of Tsukuba, 1-1-1 Tenodai, Tsukuba 305-8572, Japan.
College of General Education, Kookmin University, Seoul 02707, Korea.
Molecules. 2022 Feb 7;27(3):1111. doi: 10.3390/molecules27031111.
The present research investigates the tuber proteome of the 'medicinal' plant (abbreviated as JA) ( L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as 'kiku-imo') as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744.
本研究采用高通量蛋白质组学技术研究了（简称 JA）（L.）的块茎蛋白质组。虽然 JA 自古以来就为美洲原住民所熟知，但它在 19 世纪后期被引入欧洲，后来作为治疗糖尿病的民间疗法传播到日本（称为“菊芋”）。Genboku Takahashi 研究小组一直在研究菊芋块茎的栽培和利用，将其作为日常生活中的传统/替代药物，并研究其对降低血糖水平、HbA1c 等方面的作用（未发表的数据）。了解块茎的蛋白质成分可能有助于揭示其治疗特性，特别是与糖尿病相关的特性。使用三种商业加工的 JA 块茎产品（干粉末和干片），我们使用无标记定量蛋白质组学方法（质谱）对粉末样品进行了总蛋白提取，并首次对 JA 块茎进行了全面的蛋白质清单编制。共鉴定出 2967 个蛋白质组，经过统计学分析，并进一步使用生物信息学技术将其分类为不同的蛋白质类别。我们讨论了这些蛋白质与健康和疾病调节代谢的关联。数据可通过 ProteomeXchange 标识符 PXD030744 获取。
