Walton P E, Etherton T D, Evock C M
Endocrinology. 1986 Jun;118(6):2577-81. doi: 10.1210/endo-118-6-2577.
The effects of physiological levels of pituitary porcine GH (ppGH) and recombinant pGH (rpGH) on lipogenesis in pig adipose tissue incubated with insulin and hydrocortisone (HC) were measured in short term (2-h) incubations and after long term culture (50 h). HC (50 ng/ml) had no effect on lipogenesis in 2-h incubations; however, HC and insulin (10 ng/ml) maintained the lipogenic capacity of cultured tissue at rates comparable to those in fresh adipose tissue. Neither ppGH nor rpGH (1 and 10 ng/ml, respectively) had any effect in short term incubations. After 50 h of culture, ppGH and rpGH both directly antagonized the ability of insulin to maintain lipogenesis; however, this antagonism was markedly enhanced by HC. The present study is the first to demonstrate a direct antagonism of insulin action by ppGH and rpGH in pig adipose tissue, and a marked potentiation of this antagonism by HC. This intrinsic property of pGH may account, in part, for the decrease in adipose tissue growth rates in pigs treated chronically with pGH.
在短期（2小时）孵育以及长期培养（50小时）后，测定了生理水平的垂体猪生长激素（ppGH）和重组猪生长激素（rpGH）对与胰岛素和氢化可的松（HC）一起孵育的猪脂肪组织中脂肪生成的影响。在2小时孵育中，HC（50纳克/毫升）对脂肪生成没有影响；然而，HC和胰岛素（10纳克/毫升）使培养组织的脂肪生成能力维持在与新鲜脂肪组织相当的水平。在短期孵育中，ppGH和rpGH（分别为1和10纳克/毫升）均无任何作用。培养50小时后，ppGH和rpGH均直接拮抗胰岛素维持脂肪生成的能力；然而，HC显著增强了这种拮抗作用。本研究首次证明了ppGH和rpGH在猪脂肪组织中对胰岛素作用的直接拮抗作用，以及HC对这种拮抗作用的显著增强作用。pGH的这种内在特性可能部分解释了长期用pGH治疗的猪脂肪组织生长速率下降的原因。
