Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, 781022, India.
Advanced State Level Biotech Hub, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, 781022, India.
Vet Res Commun. 2022 Sep;46(3):799-810. doi: 10.1007/s11259-022-09900-z. Epub 2022 Feb 15.
We investigated the prevalence, antimicrobial susceptibility, antimicrobial resistance and virulence genes of Salmonella isolates recovered from humans and different species of animals. Out of 1231 samples, 88 (7.15%) Salmonella isolates were obtained, among which 21 (23.86%) belonged to Salmonella enterica subsp. enterica sero var. Weltevreden, 22 (25%) to S. Enteritidis, 16 (18.2%) to S. Typhi and 14 (15.9%) to S. Newport; 7 (7.95%) isolates were untypable. Among the 88 isolates, 65.90% showed resistance to gentamicin, 61.36% to tetracycline, 61.18% to cefotaxime, 48.86% to trimethoprim, 45.45% to ampicillin, 11.36% to ceftriaxone, 10.22% to chloramphenicol and 7.95% each to ciprofloxacin and cefepime. Most of the isolates were susceptible, with a low MIC (≤ 0.25 μg/ml) value, to cefepime, cefotaxime, ciprofloxacin, ceftriaxone and co-trimoxazole and with a moderate MIC (0.5-4 μg/ml) to ampicillin, tetracycline, gentamicin and chloramphenicol. The resistance genes bla, tetA and dfrA12 were most prevalent, irrespective of the host of origin of the isolates. While invA was used for molecular detection of Salmonella, other virulence genes, viz. sipA, sipB, sipC, stn and pagN, were also detected in all Salmonella isolates. A total of 38.64% isolates were multidrug-resistant (MDR), and various virulence genes were present among the isolated serovars. This study highlights the importance of continuous monitoring and surveillance for pathogenic Salmonella and their potential risks to both humans and animals.
我们调查了从人类和不同种类动物中分离出的沙门氏菌分离株的流行率、抗菌药物敏感性、抗菌药物耐药性和毒力基因。在 1231 个样本中，共获得 88 株沙门氏菌分离株，其中 21 株（23.86%）属于沙门氏菌肠亚种肠血清型 Weltevreden，22 株（25%）属于肠炎沙门氏菌，16 株（18.2%）属于伤寒沙门氏菌，14 株（15.9%）属于纽波特沙门氏菌；7 株（7.95%）分离株无法定型。在 88 株分离株中，65.90%对庆大霉素耐药，61.36%对四环素耐药，61.18%对头孢噻肟耐药，48.86%对甲氧苄啶耐药，45.45%对氨苄西林耐药，11.36%对头孢曲松耐药，10.22%对氯霉素耐药，7.95%对环丙沙星和头孢吡肟耐药。大多数分离株对头孢吡肟、头孢噻肟、环丙沙星、头孢曲松和复方磺胺甲噁唑敏感，MIC 值较低（≤0.25μg/ml），对氨苄西林、四环素、庆大霉素和氯霉素中度敏感，MIC 值为 0.5-4μg/ml。bla、tetA 和 dfrA12 耐药基因最为常见，而与分离株的宿主来源无关。虽然 invA 用于沙门氏菌的分子检测，但 sipA、sipB、sipC、stn 和 pagN 等其他毒力基因也在所有沙门氏菌分离株中检测到。共有 38.64%的分离株为多药耐药（MDR），不同血清型的分离株存在多种毒力基因。本研究强调了对致病性沙门氏菌及其对人类和动物潜在风险进行持续监测和监测的重要性。
