无线光遗传学激活 UCP1 非依赖性钙循环产热
Activation of UCP1-Independent Ca Cycling Thermogenesis by Wireless Optogenetics.
机构信息
Department of Molecular Endocrinology and Metabolism, Tokyo Medical and Dental University, Tokyo, Japan.
Department of Endocrinology and Metabolism, Yokohama Medical Center, Yokohama, Japan.
出版信息
Methods Mol Biol. 2022;2448:131-139. doi: 10.1007/978-1-0716-2087-8_9.
Abstract
The identification of non-canonical UCP1-independent thermogenic mechanisms offers new opportunities to target such pathways to improve metabolic health. Based on our recent studies on Ca futile cycling thermogenesis in beige fat, we applied the newly developed implantable wireless optogenetic system to activate Ca cycling in an adipocyte-specific manner without external stimuli, i.e., fat-specific cold mimetics. Here, we describe the detailed methodology and application to the prevention of obesity.
摘要
鉴定非典型 UCP1 独立的产热机制为靶向这些途径以改善代谢健康提供了新的机会。基于我们最近关于米色脂肪中钙无效循环产热的研究，我们应用新开发的可植入无线光遗传系统以非外部刺激的方式（即脂肪特异性冷模拟物）特异性地激活钙循环。在此，我们描述了详细的方法学及其在预防肥胖中的应用。
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2
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3
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