Shanghai University of Traditional Chinese Medicine, Shanghai University of Medicine and Health Sciences, Shanghai, 201203, China.
Shanghai University of Medicine and Health Sciences, Shanghai Zhoupu Hospital, Shanghai, 201318, China.
J AOAC Int. 2022 Jun 29;105(4):1175-1182. doi: 10.1093/jaoacint/qsac024.
Nuciferine is an amorphine alkaloid in lotus leaf that has anti-inflammatory, lipid-lowering and hypoglycemic effects, so the quantitation of detected nuciferine is important.
An electrochemical method was developed for nuciferine detection based on efficient catalysis by Zr-MOFs (Metal-organic frameworks).
In this work, the ratiometric electrochemical method was developed for nuciferine detection based on efficient catalysis by Zr-MOFs. UiO66 is a Zr-MOFs nanomaterial and can absorb methylene blue (MB) by electrostatic action to form UiO66-MB nanocomposite. The UiO66-MB nanocomposite can be used as an enhancer to catalyze nuciferine decomposition and a carrier to provide a two-dimensional environment for the reaction of nuciferine. Moreover, good catalytic properties of UiO66 were first time used for the detection of nuciferine.
This method has a linear detection range from 0.1 to approximately 20 μg/mL, and a low detection limit of 0.03 μg/mL (S/N=3). The recovery was from 98.1 to 102% and the RSD was from 0.45 to 3.65%, indicating that the proposed method can be applied for the analysis of real samples.
The proposed electrochemical method can be used to detect nuciferine in lotus leaves.
The ratiometric electrochemical method was used for the detection of nuciferine. The MB can be used as an internal standard for anti-interference. And, UiO66 is used to catalyze the decomposition of nuciferine. Great catalytic properties of UiO66 were first time used for the detection of nuciferine.
荷叶中的荷叶碱是一种阿朴啡生物碱，具有抗炎、降脂和降血糖作用，因此检测到的荷叶碱的定量很重要。
基于 Zr-MOFs（金属有机骨架）的高效催化作用，开发了一种用于荷叶碱检测的电化学方法。
在这项工作中，基于 Zr-MOFs 的高效催化作用，开发了一种用于荷叶碱检测的比率型电化学方法。UiO66 是一种 Zr-MOFs 纳米材料，可通过静电作用吸附亚甲基蓝（MB）形成 UiO66-MB 纳米复合材料。UiO66-MB 纳米复合材料可用作催化剂，促进荷叶碱分解，并提供二维反应环境。此外，首次将 UiO66 的良好催化性能用于荷叶碱的检测。
该方法的线性检测范围为 0.1 至约 20μg/mL，检测限低至 0.03μg/mL（S/N=3）。回收率为 98.1%至 102%，RSD 为 0.45%至 3.65%，表明该方法可用于实际样品的分析。
提出的电化学方法可用于检测荷叶中的荷叶碱。
该比率型电化学方法用于检测荷叶碱。MB 可作为抗干扰的内标。并且，UiO66 用于催化荷叶碱的分解。首次将 UiO66 的优异催化性能用于荷叶碱的检测。
