School of Biotechnology, Jiangsu University of Science and Technology, Jiangsu Province, Zhenjiang 212008, China; Jiangsu Key Laboratory of Sericulture Biology and Biotechnology, Jiangsu Province, Zhenjiang 212008, China.
School of Biotechnology, Jiangsu University of Science and Technology, Jiangsu Province, Zhenjiang 212008, China.
Food Chem. 2022 Jul 30;383:132400. doi: 10.1016/j.foodchem.2022.132400. Epub 2022 Feb 10.
Silkworm (Bombyx mori) was used to explore the anti-aging ability of Gamma-aminobutyric acid (GABA) and its potential mechanism. 0.086∼8.6 mM GABA solutions were sprayed on mulberry leaves to raise silkworms. The results showed that GABA increased the fecundity of adult silkworms. And in the larva silkworms on the 3th day of the 5th instar, GABA significantly reduced the trehalose content in the hemolymph, the triglycerides and glycogen levels in the fat body, while sharply increased the NAD/NADH level in the fat body. GABA significantly increased the GSH content and activities of SOD and CAT, while reduced the level of MDA. Furthermore, GABA reduced the mRNA expression of BmRpd3, Bmchico and BmAkh2, while increased the mRNA expression of BmAMPK. In summary, GABA has anti-aging potential by playing roles in energy homeostasis, reducing carbohydrate and lipid level, increasing anti-oxidative capacity, and regulating mRNA expression of longevity-related genes.
家蚕（Bombyx mori）被用来探索γ-氨基丁酸（GABA）的抗衰老能力及其潜在机制。将 0.086∼8.6 mM 的 GABA 溶液喷洒在桑叶上以饲养家蚕。结果表明，GABA 提高了成虫的繁殖力。并且在 5 龄幼虫第 3 天，GABA 显著降低了血液中的海藻糖含量、脂肪体中的甘油三酯和糖原水平，而脂肪体中的 NAD/NADH 水平急剧增加。GABA 显著增加了 GSH 含量以及 SOD 和 CAT 的活性，同时降低了 MDA 水平。此外，GABA 降低了 BmRpd3、Bmchico 和 BmAkh2 的 mRNA 表达，而增加了 BmAMPK 的 mRNA 表达。综上所述，GABA 通过在能量平衡、降低碳水化合物和脂质水平、增加抗氧化能力以及调节与长寿相关基因的 mRNA 表达方面发挥作用，具有抗衰老潜力。
