Lian Meifei, Han Yu, Sun Binbin, Xu Ling, Wang Xiaofeng, Ni Bing, Jiang Wenbo, Qiao Zhiguang, Dai Kerong, Zhang Xiuyin
Department of Prosthodontics, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; Clinical and Translational Research Center for 3D Printing Technology, Medical 3D Printing Innovation Research Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong university School of Medicine, Shanghai 200125, China.
Acta Biomater. 2020 Dec;118:83-99. doi: 10.1016/j.actbio.2020.08.017. Epub 2020 Aug 25.
The guided bone regeneration (GBR) concept has been extensively utilized to treat maxillofacial bone defects in clinical practice. However, the repair efficacy of currently available GBR membranes is often compromised by their limited bone regeneration potential and deficient antibacterial activity. In this study, inspired by the bi-layered structure design of the commonly used Bio-Gidemembrane, we designed and fabricated a new kind of multifunctional bi-layered "GBR scaffold" combining solution electrospinning writing (SEW) and solution electrospinning (SES) techniques using a single SEW printer. Copper-loaded mesoporous silica nanoparticles (Cu@MSNs) were incorporated into the poly(lactic-co-glycolic acid)/gelatin (PLGA/Gel, denoted as PG) fiber matrix to construct a composite PG-Cu@MSNs fibrous scaffold. The obtained GBR scaffold consisted of a loose and porous SEW layer to support and facilitate bone ingrowth, and a dense and compact SES layer to resist non-osteoblast interference. The resulting enhanced mechanical properties, coordinated degradation profile, and facile preparation procedure imparted the composite scaffold with good clinical feasibility. In vitro biological experiments indicate that the PG-Cu@MSNs composite scaffold exhibited favorable osteogenic and antibacterial properties. Furthermore, an in vivo rat periodontal defect model further confirmed the promising bone regeneration efficacy of the PG-Cu@MSNs scaffold. In conclusion, the developed electrowritten Cu@MSNs-incorporated bi-layered scaffold with hierarchical architecture and concurrent osteogenic and antibacterial functions may hold great potential for application in GBR.
引导骨再生（GBR）概念在临床实践中已被广泛用于治疗颌面部骨缺损。然而，目前可用的GBR膜的修复效果常常因其有限的骨再生潜力和不足的抗菌活性而受到影响。在本研究中，受常用的Bio-Gide膜的双层结构设计启发，我们使用单台溶液电纺书写（SEW）打印机，设计并制造了一种结合溶液电纺书写（SEW）和溶液静电纺丝（SES）技术的新型多功能双层“GBR支架”。将载铜介孔二氧化硅纳米颗粒（Cu@MSNs）掺入聚乳酸-乙醇酸共聚物/明胶（PLGA/Gel，简称PG）纤维基质中，构建复合PG-Cu@MSNs纤维支架。所获得的GBR支架由疏松多孔的SEW层组成，以支持并促进骨向内生长，以及致密紧实的SES层，以抵抗非成骨细胞的干扰。由此增强的力学性能、协调的降解特性以及简便的制备过程赋予了复合支架良好的临床可行性。体外生物学实验表明，PG-Cu@MSNs复合支架表现出良好的成骨和抗菌性能。此外，体内大鼠牙周缺损模型进一步证实了PG-Cu@MSNs支架具有良好的骨再生效果。总之，所开发的具有分层结构以及同时具备成骨和抗菌功能的电纺含Cu@MSNs双层支架在GBR应用中可能具有巨大潜力。
