Division of Rheumatology, Cincinnati Children's Hospital Medical Center and Department of Pediatrics, University of Cincinnati, Cincinnati, OH.
Division of Ophthalmology, Cincinnati Children's Hospital Medical Center and Department of Pediatrics, University of Cincinnati, Cincinnati, OH.
Ocul Immunol Inflamm. 2021;29(7-8):1616-1620. doi: 10.1080/09273948.2020.1758731. Epub 2020 May 28.
Biomarkers for juvenile idiopathic arthritis-associated uveitis (JIA-U) are needed. We aimed to measure inflammatory biomarkers in tears as a non-invasive method to identify biomarkers of uveitis activity.
Tears were collected from children with JIA-U (n=20) and pediatric controls (n=20) using Schirmer strips. S100A8, A9, A12, IL-18, IL-8, IP-10, MCP-1, RANTES, and sICAM-1 were measured by ELISA and Luminex assays. Levels of biomarkers were compared between children with JIA-U and controls, and active and inactive JIA-U.
IL-8, sICAM-1, and S100A12 levels were similar between JIA-U and controls, but differed by activity. Active JIA-U had significantly increased S100A12 compared to inactive JIA-U (mean 27,722.5 pg/ML (SE 1.3) vs. 5,937.2 (1.3), p=0.002), IL-8 (73.5 [1.2] vs. 36.2 [1.2], p=0.009), and sICAM-1 (15,822.7 [1.2) vs. 8,778.0 [1.6], p=0.024).
We detected inflammatory biomarkers non-invasively in tears of children with JIA-U. IL-8, sICAM-1, and S100A12 are potential biomarkers for uveitis activity.
需要用于幼年特发性关节炎相关葡萄膜炎（JIA-U）的生物标志物。我们旨在通过测量泪液中的炎症生物标志物，作为一种非侵入性方法来鉴定葡萄膜炎活动的生物标志物。
使用 Schirmer 条从患有 JIA-U（n=20）和儿科对照（n=20）的儿童中收集泪液。通过 ELISA 和 Luminex 测定法测量 S100A8、A9、A12、IL-18、IL-8、IP-10、MCP-1、RANTES 和 sICAM-1 的水平。比较 JIA-U 患儿与对照组以及活动性和非活动性 JIA-U 患儿之间生物标志物的水平。
JIA-U 和对照组之间的 IL-8、sICAM-1 和 S100A12 水平相似，但活性不同。与非活动性 JIA-U 相比，活动性 JIA-U 的 S100A12 水平显著升高（平均 27722.5 pg/ml（SE 1.3）比 5937.2（1.3），p=0.002）、IL-8（73.5[1.2]比 36.2[1.2]，p=0.009）和 sICAM-1（15822.7[1.2]比 8778.0[1.6]，p=0.024）。
我们在患有 JIA-U 的儿童的泪液中无创地检测到炎症生物标志物。IL-8、sICAM-1 和 S100A12 可能是葡萄膜炎活动的生物标志物。
