Stevens Victoria L, Hoover Elise, Wang Ying, Zanetti Krista A
Behavioral and Epidemiology Research Group, American Cancer Society, Atlanta, GA 30303, USA.
Epidemiology and Genomics Research Program, Division of Cancer Control and Population Sciences, National Cancer Institute, Rockville, MD 20850, USA.
Metabolites. 2019 Jul 25;9(8):156. doi: 10.3390/metabo9080156.
Metabolomics provides a comprehensive assessment of numerous small molecules in biological samples. As it integrates the effects of exogenous exposures, endogenous metabolism, and genetic variation, metabolomics is well-suited for studies examining metabolic profiles associated with a variety of chronic diseases. In this review, we summarize the studies that have characterized the effects of various pre-analytical factors on both targeted and untargeted metabolomic studies involving human plasma, serum, and urine and were published through 14 January 2019. A standardized protocol was used for extracting data from full-text articles identified by searching PubMed and EMBASE. For plasma and serum samples, metabolomic profiles were affected by fasting status, hemolysis, collection time, processing delays, particularly at room temperature, and repeated freeze/thaw cycles. For urine samples, collection time and fasting, centrifugation conditions, filtration and the use of additives, normalization procedures and multiple freeze/thaw cycles were found to alter metabolomic findings. Consideration of the effects of pre-analytical factors is a particularly important issue for epidemiological studies where samples are often collected in nonclinical settings and various locations and are subjected to time and temperature delays prior being to processed and frozen for storage.
代谢组学可对生物样本中的众多小分子进行全面评估。由于它整合了外源性暴露、内源性代谢和基因变异的影响，因此代谢组学非常适合用于研究与各种慢性疾病相关的代谢谱。在本综述中，我们总结了截至2019年1月14日发表的有关各种分析前因素对涉及人血浆、血清和尿液的靶向和非靶向代谢组学研究影响的研究。我们使用标准化方案从通过搜索PubMed和EMBASE识别出的全文文章中提取数据。对于血浆和血清样本，代谢组学谱受禁食状态、溶血、采集时间、处理延迟（尤其是在室温下）以及反复冻融循环的影响。对于尿液样本，发现采集时间和禁食、离心条件、过滤和添加剂的使用、标准化程序以及多次冻融循环会改变代谢组学研究结果。对于流行病学研究而言，考虑分析前因素的影响是一个特别重要的问题，因为在这些研究中，样本通常在非临床环境和不同地点采集，并且在处理和冷冻保存之前会经历时间和温度延迟。
