Gimenez H B, Hardman N, Keir H M, Cash P
J Gen Virol. 1986 May;67 ( Pt 5):863-70. doi: 10.1099/0022-1317-67-5-863.
Three hybridoma antibodies, prepared against the RSN-2 strain of human respiratory syncytial (RS) virus, have been used to identify antigenic variation between 41 isolates of RS virus collected from widely separated geographical regions over a period of 29 years. One antibody was directed against an antigenic site on the virus fusion protein, VP70. This site was shared by 21 virus isolates tested and its recognition by the antibody was sensitive to the presence of 2-mercaptoethanol. The remaining two antibodies used react against the virus phosphoprotein, VPP32. Two independent sites were recognized on VPP32 by these antibodies. One antibody reacted with all of the virus isolates screened while the second reacted with only 21 out of the 41 virus isolates. On the basis of the variable epitope, two antigenic types of human RS virus were identified. The distribution of each antigenic group among 28 RS virus isolates from the Grampian Region, north-east Scotland, collected between 1982 and 1984 was determined. The reactivity of these antibodies was examined using immunofluorescence staining and by immunoblotting; the latter technique also revealed that the electrophoretic mobility of VPP32 varied in parallel with the variable antigenic site.
三种针对人类呼吸道合胞（RS）病毒RSN - 2株制备的杂交瘤抗体，已被用于鉴定在29年期间从广泛分离的地理区域收集的41株RS病毒之间的抗原变异。一种抗体针对病毒融合蛋白VP70上的一个抗原位点。21株受试病毒分离株共享该位点，并且抗体对其识别对2 - 巯基乙醇的存在敏感。所使用的其余两种抗体与病毒磷蛋白VPP32发生反应。这些抗体在VPP32上识别出两个独立的位点。一种抗体与所有筛选的病毒分离株发生反应，而另一种仅与41株病毒分离株中的21株发生反应。基于可变表位，鉴定出两种抗原类型的人类RS病毒。确定了1982年至1984年间从苏格兰东北部格兰扁地区收集的28株RS病毒分离株中每个抗原组的分布。使用免疫荧光染色和免疫印迹法检测了这些抗体的反应性；后一种技术还表明，VPP32的电泳迁移率与可变抗原位点平行变化。
