Lee M, Pietras D F, Nemeroff M E, Corstanje B J, Field L J, Bruenn J A
J Virol. 1986 May;58(2):402-7. doi: 10.1128/JVI.58.2.402-407.1986.
We have completely sequenced a defective interfering viral double-stranded RNA (dsRNA) from the Saccharomyces cerevisiae virus. This RNA (S14) is a simple internal deletion of its parental dsRNA, M1, of 1.9 kilobases. The 5' 964 bases of the M1 plus strand encode the type 1 killer toxin of the yeast. S14 is 793 base pairs (bp) long, with 253 bp from the 5' region of its parental plus strand and 540 bp from the 3' region. All three defective interfering RNAs derived from M1 that have been characterized so far preserve a large 3' region, which includes five repeats of a rotationally symmetrical 11-bp consensus sequence. This 11-bp sequence is not present in the 5' 1 kilobase of the parental RNA or in any of the sequenced regions of unrelated yeast viral dsRNAs, but it is present in the 3' region of the plus strand of another yeast viral dsRNA, M2, that encodes the type 2 killer toxin. The 3' region of 550 bases of the M1 plus strand, previously only partially sequenced, reveals no large open reading frames. Hence only about half of M1 appears to have a coding function.
我们已经对来自酿酒酵母病毒的一种缺陷干扰病毒双链RNA（dsRNA）进行了全序列测定。这种RNA（S14）是其亲本dsRNA M1的一个简单内部缺失，缺失长度为1.9千碱基。M1正链的5'端964个碱基编码酵母的1型杀伤毒素。S14长793个碱基对（bp），其253 bp来自亲本正链的5'区域，540 bp来自3'区域。到目前为止，已鉴定的源自M1的所有三种缺陷干扰RNA都保留了一个大的3'区域，该区域包含一个11 bp旋转对称共有序列的五个重复。这个11 bp序列在亲本RNA的5'端1千碱基中不存在，在无关酵母病毒dsRNA的任何测序区域中也不存在，但它存在于另一种编码2型杀伤毒素的酵母病毒dsRNA M2正链的3'区域。M1正链550个碱基的3'区域，之前仅部分测序，未发现大的开放阅读框。因此，M1似乎只有大约一半具有编码功能。
