Näslund M, Kláŝterská I, Kolman A, Ehrenberg L
Mutat Res. 1986 Jun;161(1):1-7. doi: 10.1016/0027-5107(86)90094-1.
5'-Deoxy-5'-(methylthio)adenosine (MTA) alone and in combination with methionine was found to increase frequencies of background HGPRT mutation and SCE in V79 Chinese hamster cells. The same agents exert a comutagenic action on these effects as well as upon mutation induction in Escherichia coli stain AB1157 (but not in its non-adaptable derivative ada-6) following treatment with N-methyl-N-nitrosourea (MNU). MTA plus methionine also enhanced the lethal action of MNU on hamster cells. The effects observed may tentatively be ascribed to hypomethylation due to inhibition of DNA methylase by MTA.
研究发现，5'-脱氧-5'-(甲硫基)腺苷（MTA）单独使用以及与蛋氨酸联合使用时，会增加V79中国仓鼠细胞中背景次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）突变和姐妹染色单体交换（SCE）的频率。在用N-甲基-N-亚硝基脲（MNU）处理后，这些试剂对这些效应以及对大肠杆菌菌株AB1157（但不对其非适应性衍生物ada-6）的突变诱导均具有协同诱变作用。MTA加蛋氨酸也增强了MNU对仓鼠细胞的致死作用。观察到的这些效应可能暂时归因于MTA对DNA甲基化酶的抑制导致的低甲基化。
