State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province, China.
College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai Province, China.
PeerJ. 2022 Feb 1;10:e12754. doi: 10.7717/peerj.12754. eCollection 2022.
Maxim., an alpine plant, is traditionally used for several medicinal purposes. Here, both chloroplast DNA sequences and nuclear low copy sequence markers were used to investigate the genetic diversity and population structure of . Materials were collected from thirteen localities in the northeast Qinghai-Tibet Plateau (QTP) and adjacent highlands and advanced analytical toolkits were used to access their origin and range shifts. The results revealed a higher level of population differentiation based on chloroplast DNA (cpDNA) concatenated sequences compared with the nuclear DNA sequences ( = 0.654 for cpDNA, = 0.398 for AT103), indicating that pollen flow was still extensive in . A decline in haplotype variation was observed from the plateau edge and adjoining highlands toward the platform of the QTP. The hypothesis "dispersal into the QTP," proposing that experienced migration from the plateau edge and adjacent highlands to the platform, was supported. These results were in line with the hypothesis that multiple refugia exist on the plateau edge and adjacent highlands rather than on the plateau platform. Our unimodal mismatch distribution, star-like network supported a recent expansion in .
獐牙菜是一种高山植物，传统上被用于多种药用目的。在这里，我们同时使用叶绿体 DNA 序列和核低拷贝序列标记来研究青藏高原东北部及其毗邻高地獐牙菜的遗传多样性和种群结构。我们从青藏高原东北部的十三个地点和毗邻的高地收集了材料，并使用先进的分析工具来确定它们的起源和分布范围的变化。结果表明，基于叶绿体 DNA（cpDNA）串联序列的种群分化程度高于核 DNA 序列（cpDNA 为 0.654，AT103 为 0.398），表明花粉流在獐牙菜中仍然很广泛。从高原边缘和毗邻的高地到青藏高原平台，观察到单倍型变异的减少。“向青藏高原扩散”的假说，即獐牙菜从高原边缘和毗邻的高地向高原平台扩散，得到了支持。这些结果与高原边缘和毗邻高地存在多个避难所的假说一致，而不是在高原平台上。我们的单峰不匹配分布、星形网络支持了獐牙菜的近期扩张。
