Thorp B H, Lynch M, Duff S R
Res Vet Sci. 1986 Mar;40(2):236-40.
A method is described which enables visualisation of the blood supply in developing avian long bones, followed by the preparation of undecalcified histological sections from the same material. The circulatory system was perfused with a solution of fixative, dye and barium sulphate. The skeletal tissue was cleared in plastic resin before embedding and tissue blocks were cut into 1 mm slabs. The vascular canals were then examined with a dissecting microscope. Slabs were re-embedded in resin and 5 microns sections cut for routine undecalcified histological staining. Focal areas of delayed endochondral ossification were demonstrated in slabs prepared from the proximal ends of femora of White Leghorns. These lesions are considered a less severe form of the dyschondroplastic condition occurring in broilers.
本文描述了一种方法，该方法能够观察发育中的禽类长骨的血液供应情况，随后用相同材料制备不脱钙组织切片。用含有固定剂、染料和硫酸钡的溶液灌注循环系统。在包埋前，骨骼组织用塑料树脂进行透明处理，然后将组织块切成1毫米厚的薄片。然后用解剖显微镜检查血管管道。将薄片重新包埋在树脂中，切成5微米厚的切片，进行常规的不脱钙组织学染色。在白来航母鸡股骨近端制备的薄片中发现了软骨内骨化延迟的局部区域。这些病变被认为是肉鸡发生的软骨发育不良状况的一种不太严重的形式。
